Rabbit CEBP alpha/CEBPA Polyclonal Antibody | anti-CEBPA antibody

Anti-CEBP alpha/CEBPA Antibody, Rabbit Polyclonal

Cat. Num. AAA254208

• Gene Names: CEBPA; CEBP; C/EBP-alpha
• Reactivity: Human; Predicted to React with: Mouse, Rat (Species predicted to react based on 100% sequence homology)
• Applications: Immunoprecipitation, Western Blot
• Purity: Protein A & Antigen Affinity
• Synonyms: CEBP alpha/CEBPA, Antibody; Anti-CEBP alpha/CEBPA Antibody, Rabbit Polyclonal; CEBPA Antibody, Rabbit PAb, Antigen Affinity Purified; CCAAT/enhancer binding protein alpha; Anti-C/EBP-alpha Antibody; Anti-CEBP Antibody; anti-CEBPA antibody

• Host: Rabbit
• Reactivity: Human; Predicted to React with: Mouse, Rat (Species predicted to react based on 100% sequence homology)
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Specificity: Human CEBP alpha/CEBPA
• Purity/Purification: Protein A & Antigen Affinity
• Form/Format: Liquid; 0.2um filtered solution in PBS

• Applicable Applications for anti-CEBPA antibody: IP (Immunoprecipitation), WB (Western Blot)
• Immunogen: A synthetic peptide corresponding to the C-terminus of the human CEBPA
• Conjugation: Unconjugated
• Preparation: Produced in rabbits immunized with purified, a synthetic peptide corresponding to the C-terminus of the human CEBPA. Huamn CEBPA specific IgG was purified by Huamn CEBPA affinity chromatography.
• Preparation and Storage: This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Preservative-Free. Avoid repeated freeze-thaw cycles.

WB (Western Blot)

(Anti-CEBPA rabbit polyclonal antibody at 1:500 dilutionLane A: U937 Whole Cell LysateLane B: Jurkat Whole Cell LysateLane C: MCF7 Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.Developed using the Odyssey technique.Performed under reducing conditions.Predicted band size:43 kDaObserved band size:37 kDa(We are unsure as to the identity of these extra bands.))

IP (Immunoprecipitation)

(CEBPA was immunoprecipitated using:Lane A:0.5 mg HepG2 Whole Cell Lysate2 uL anti-CEBPA rabbit polyclonal antibody and 15 ul of 50 % Protein G agarose.Primary antibody:Anti-CEBPA rabbit polyclonal antibody,at 1:200 dilutionSecondary antibody:Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilutionDeveloped using the odssey technique.Performed under reducing conditions.Predicted band size: 38 kDaObserved band size: 38 kDa)

Related Product Information for anti-CEBPA antibody

The CCAAT/enhancer-binding protein-alpha (CEBPA) is a lineage-specific transcription factor in the hematopoietic system. The presence of biallelic CEBPA mutations is a favourable prognostic feature in acute myeloid leukaemia (AML). CEBPA mutations are currently identified through conventional capillary sequencing (CCS). Mutations in CCAAT/enhancer binding protein alpha (CEBPA) occur in 5-10% of cases of acute myeloid leukemia. CCAAT/enhancer binding protein alpha gene (CEBPA) is an important transcription factor in maintenance of differentiation of granulocyte series of hematopoietic system. It plays a key role in regulating cell proliferation and differentiation.

Product Categories/Family for anti-CEBPA antibody

IL17 signaling pathway

NCBI and Uniprot Product Information

• NCBI GI #: 28872794
• NCBI GeneID: 1050
• NCBI Accession #: NP_004355.2
• NCBI GenBank Nucleotide #: NM_004364.3
• UniProt Accession #: P49715; P78319; Q05CA4; A7LNP2
• Molecular Weight: 37,561 Da
• NCBI Official Full Name: CCAAT/enhancer-binding protein alpha
• NCBI Official Synonym Full Names: CCAAT/enhancer binding protein (C/EBP), alpha
• NCBI Official Symbol: CEBPA
• NCBI Official Synonym Symbols: CEBP; C/EBP-alpha
• NCBI Protein Information: CCAAT/enhancer-binding protein alpha
• UniProt Protein Name: CCAAT/enhancer-binding protein alpha
• UniProt Gene Name: CEBPA
• UniProt Synonym Gene Names: C/EBP alpha
• UniProt Entry Name: CEBPA_HUMAN