Rabbit anti-Human CD50 Polyclonal Antibody | anti-ICAM3 antibody

Anti-CD50 Antibody, Rabbit Polyclonal

Cat. Num. AAA254834

• Gene Names: ICAM3; CD50; CDW50; ICAM-R
• Reactivity: Human
• Applications: Immunoprecipitation, ELISA, Western Blot
• Purity: Protein A & Antigen Affinity
• Synonyms: CD50, Antibody; Anti-CD50 Antibody, Rabbit Polyclonal; CD50/ICAM-3 Antibody, Rabbit PAb, Antigen Affinity Purified; intercellular adhesion molecule 3; Anti-CD50 Antibody; Anti-CDW50 Antibody; Anti-ICAM-3 Antibody; Anti-ICAM-R Antibody; anti-ICAM3 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Specificity: Human ICAM3/CD50
• Purity/Purification: Protein A & Antigen Affinity
• Form/Format: Liquid; 0.2um filtered solution in PBS

• Applicable Applications for anti-ICAM3 antibody: IP (Immunoprecipitation), ELISA, WB (Western Blot)
• Immunogen: Recombinant Human ICAM3/CD50 protein
• Conjugation: Unconjugated
• Preparation: Produced in rabbits immunized with purified, recombinant Human ICAM3/CD50 (rh ICAM3/CD50). ICAM3/CD50 specific IgG was purified by Human ICAM3/CD50 affinity chromatography.
• Preparation and Storage: This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Preservative-Free. Avoid repeated freeze-thaw cycles.

IP (Immunoprecipitation)

(ICAM3 was immunoprecipitated using:Lane A:0.5 mg THP-1 Whole Cell Lysate2 uL anti-ICAM3 rabbit polyclonal antibody and 15 ul of 50 % Protein G agarose.Primary antibody:Anti-ICAM3 rabbit polyclonal antibody,at 1:200 dilutionSecondary antibody:Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilutionDeveloped using the odssey technique.Performed under reducing conditions.Predicted band size: 60 kDaObserved band size: 170 kDa)

WB (Western Blot)

(Anti-ICAM3 rabbit polyclonal antibody at 1:500 dilutionLane A: HL60 Membrane LysateLysates/proteins at 20 ug per lane.SecondaryGoat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.Developed using the Odyssey technique.Performed under reducing conditions.Predicted band size:60 kDaObserved band size:150 kDa)

Related Product Information for anti-ICAM3 antibody

The protein ICAM-3, also known as CD50, is a member of the intercellular adhesion molecule (ICAM) family consisting of three members. It is a DC-SIGN ligand that is constitutively expressed on resting leukocytes and is thus an important molecule for the first immune response. ICAM-3 comprises five immunoglobulin-like domains and binds LFA-1 through its two N-terminal domains. It functions not only as an adhesion molecule but also as a potent signaling molecule. ICAM-3 binds to LFA-1 on antigen-presenting cells (APC) stabilizing the T cell-APC interaction, facilitating signaling through the CD3/TCR complex. However, recent evidence using cultured and transformed T cells suggests ICAM-3 may also function in signaling. It has been reported that the CD50 molecule can play a role in developing functionally mature T lymphocytes and its expression increases during the maturation process of T lymphocytes. Also, the interactions of ICAM-3 and LFA-1 facilitate HIV-1-induced virological synapse formation between T cells. ICAM-3 is associated with an increase in cellular radio-resistance and cancer cell proliferation. It could be considered as a candidate for anti-cancer drug development and as a cancer marker.

References

Berney SM, et al. (1999) ICAM-3 (CD50) cross-linking augments signaling in CD3-activated peripheral human T lymphocytes. J Leukoc Biol. 65(6): 867-74.van Buul JD, et al. (2004) ICAM-3 activation modulates cell-cell contacts of human bone marrow endothelial cells. J Vasc Res. 41(1): 28-37.Sugino H. (2005) ICAM-3, a ligand for DC-SIGN, was duplicated from ICAM-1 in mammalian evolution, but was lost in the rodent genome. FEBS Lett. 579(13): 2901-6.Park JK, et al. (2010) ICAM-3 enhances the migratory and invasive potential of human non-small cell lung cancer cells by inducing MMP-2 and MMP-9 via Akt and CREB. Int J Oncol. 36(1): 181-92.

NCBI and Uniprot Product Information

• NCBI GI #: 167466207
• NCBI GeneID: 3385
• NCBI Accession #: NP_002153.2
• NCBI GenBank Nucleotide #: NM_002162.3
• UniProt Accession #: P32942; Q6PD68
• Molecular Weight: 59,541 Da
• NCBI Official Full Name: intercellular adhesion molecule 3
• NCBI Official Synonym Full Names: intercellular adhesion molecule 3
• NCBI Official Symbol: ICAM3
• NCBI Official Synonym Symbols: CD50; CDW50; ICAM-R
• NCBI Protein Information: intercellular adhesion molecule 3; ICAM-3; intercellular adhesion molecule-3
• UniProt Protein Name: Intercellular adhesion molecule 3
• UniProt Gene Name: ICAM3
• UniProt Synonym Gene Names: ICAM-3
• UniProt Entry Name: ICAM3_HUMAN