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IHC (Immunohistchemistry-Paraffin)
(Immunochemical staining CD21 in cynomolgus lymph node with rabbit polyclonal antibody at 1:1000 dilution, formalin-fixed paraffin embedded sections.)
Rabbit CD21 Polyclonal Antibody | anti-CD21 antibody
Anti-CD21 Antibody, Rabbit Polyclonal
Reactivity
Human, Cynomolgus(Species predicted to react based on 100% sequence homology)
Applications
Western Blot, ELISA, Immunoprecipitation, Immunohistochemistry
Purity
Protein A & Antigen Affinity
Synonyms
CD21, Antibody; Anti-CD21 Antibody, Rabbit Polyclonal; complement component (3d/Epstein Barr virus) receptor 2; Anti-C3DR Antibody; Anti-CD21 Antibody; Anti-CR Antibody; Anti-CVID7 Antibody; Anti-SLEB9 Antibody; anti-CD21 antibody
Host
Rabbit
Reactivity
Human, Cynomolgus
(Species predicted to react based on 100% sequence homology)
(Species predicted to react based on 100% sequence homology)
Clonality
Polyclonal
Isotype
Rabbit IgG
Purity/Purification
Protein A & Antigen Affinity
Form/Format
Liquid; 0.2um filtered solution in PBS
Applicable Applications for anti-CD21 antibody
Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP), Immunohistochemistry-Paraffin (IHC-P)
Application Notes
WB: 1:500-1:2000
ELISA: 1:5000-1:10000
IHC-P: 1:500-1:2000
IP: 0.5-2uL/mg of lysate
ELISA: 1:5000-1:10000
IHC-P: 1:500-1:2000
IP: 0.5-2uL/mg of lysate
Immunogen
Recombinant Human CD21 Protein
Preparation
Produced in rabbits immunized with purified, recombinant Human CD21 (rh CD21). CD21 specific IgG was purified by Human CD21 affinity chromatography.
Preparation and Storage
This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Preservative-Free. Avoid repeated freeze-thaw cycles.
IHC (Immunohistchemistry-Paraffin)
(Immunochemical staining CD21 in cynomolgus lymph node with rabbit polyclonal antibody at 1:1000 dilution, formalin-fixed paraffin embedded sections.)
IHC (Immunohistchemistry-Paraffin)
(Immunochemical staining CD21 in cynomolgus lymph node with rabbit polyclonal antibody at 1:1000 dilution, formalin-fixed paraffin embedded sections.)
IHC (Immunohistochemistry-Paraffin)
(Immunochemical staining CD21 in cynomolgus spleen with rabbit polyclonal antibody at 1:1000 dilution, formalin-fixed paraffin embedded sections.)
IHC (Immunohistochemistry-Paraffin)
(Immunochemical staining CD21 in cynomolgus spleen with rabbit polyclonal antibody at 1:1000 dilution, formalin-fixed paraffin embedded sections.)
IP (Immunoprecipitation)
(CD21 was immunoprecipitated using:Lane A:0.5 mg Daudi Whole Cell Lysate4µL anti-CD21 rabbit polyclonal antibody and 60 ug of Immunomagnetic beads Protein A/G.Primary antibody:Anti-CD21 rabbit polyclonal antibody,at 1:100 dilutionSecondary antibody:Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilutionDeveloped using the ECL technique.Performed under reducing conditions.Predicted band size: 113 kDaObserved band size :155 kDa)
IP (Immunoprecipitation)
(CD21 was immunoprecipitated using:Lane A:0.5 mg Daudi Whole Cell Lysate4µL anti-CD21 rabbit polyclonal antibody and 60 ug of Immunomagnetic beads Protein A/G.Primary antibody:Anti-CD21 rabbit polyclonal antibody,at 1:100 dilutionSecondary antibody:Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilutionDeveloped using the ECL technique.Performed under reducing conditions.Predicted band size: 113 kDaObserved band size :155 kDa)
IHC (Immunohistochemistry-Paraffin)
(Immunochemical staining CD21 in human lymph node with rabbit polyclonal antibody at 1:1000 dilution, formalin-fixed paraffin embedded sections.)
IHC (Immunohistochemistry-Paraffin)
(Immunochemical staining CD21 in human lymph node with rabbit polyclonal antibody at 1:1000 dilution, formalin-fixed paraffin embedded sections.)
IHC (Immunohistochemistry-Paraffin)
(Immunochemical staining CD21 in human tonsil with rabbit polyclonal antibody at 1:1000 dilution, formalin-fixed paraffin embedded sections.)
IHC (Immunohistochemistry-Paraffin)
(Immunochemical staining CD21 in human tonsil with rabbit polyclonal antibody at 1:1000 dilution, formalin-fixed paraffin embedded sections.)
WB (Western Blot)
(Anti-CD21 rabbit polyclonal antibody at 1:500 dilutionLane A: Raji Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution.Developed using the ECL technique.Performed under reducing conditions.Predicted band size:113 kDaObserved band size:160 kDa)
WB (Western Blot)
(Anti-CD21 rabbit polyclonal antibody at 1:500 dilutionLane A: Raji Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution.Developed using the ECL technique.Performed under reducing conditions.Predicted band size:113 kDaObserved band size:160 kDa)
Related Product Information for anti-CD21 antibody
The cluster of differentiation (CD) system is commonly used as cell markers in Immunophenotyping. Different kinds of cells in the immune system can be identified through the surface CD molecules associating with the immune function of the cell. There are more than 320 CD unique clusters and subclusters have been identified. Some of the CD molecules serve as receptors or ligands important to the cell through initiating a signal cascade which then alter the behavior of the cell. Some CD proteins do not take part in cell signal process but have other functions such as cell adhesion. CD21, also known as Complement component (3d/Epstein Barr virus) receptor 2 and CR2, is a member of the CD system and is a protein involved in complement system. CD21 is present on all mature B-cells and some T-cells and follicular dendritic cells. CD21 on mature B-cells form a complex called the B cell receptor complex with two other membrane proteins, CD19 and CD81. CD21 has a function in the complement system through serving as the cellular receptor specific for ligands such as C3 and C4 which can be attached to foreign macromolecules in order to remove or uptake them. This results in B-cells having enhanced response to the antigen.
References
Zola H, et al. (2007) CD molecules 2006-human cell differentiation molecules. J Immunol Methods. 318 (1-2): 1-5.Ho IC, et al. (2009) GATA3 and the T-cell lineage: essential functions before and after T-helper-2-cell differentiation. Nat Rev Immunol. 9 (2): 125-35.Matesanz-Isabel J, et al. (2011) New B-cell CD molecules.
Immunology Letters.134 (2): 104-12.Joseph M, et al. (1989) Structure and Function of the Complement Receptors, CR1 (CD35) and CR2 (CD21). Advanced in immunology. 46: 183-219.
Aubry JP, et al. (1992) CD21 is a ligand for CD23 and regulates IgE production. Nature 358: 505-7.
Immunology Letters.134 (2): 104-12.Joseph M, et al. (1989) Structure and Function of the Complement Receptors, CR1 (CD35) and CR2 (CD21). Advanced in immunology. 46: 183-219.
Aubry JP, et al. (1992) CD21 is a ligand for CD23 and regulates IgE production. Nature 358: 505-7.
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Product Notes
The CD21 (Catalog #AAA27778) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-CD21 Antibody, Rabbit Polyclonal reacts with Human, Cynomolgus (Species predicted to react based on 100% sequence homology) and may cross-react with other species as described in the data sheet. AAA Biotech's CD21 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP), Immunohistochemistry-Paraffin (IHC-P). WB: 1:500-1:2000 ELISA: 1:5000-1:10000 IHC-P: 1:500-1:2000 IP: 0.5-2uL/mg of lysate. Researchers should empirically determine the suitability of the CD21 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CD21, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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