Rabbit anti-Human BCORL1 Polyclonal Antibody | anti-BCORL1 antibody

BCORL1 Antibody (N-term)

Cat. Num. AAA28773

• Gene Names: BCORL1; BCoR-L1; CXorf10
• Reactivity: Human
• Applications: Flow Cytometry, Functional Assay, ELISA, Western Blot, Immunohistochemistry
• Purity: This antibody is purified through a protein A column, followed by peptide affinity purification.
• Synonyms: BCORL1, Antibody; BCORL1 Antibody (N-term); BCL-6 corepressor-like protein 1; BCoR-L1; BCoR-like protein 1; BCORL1; anti-BCORL1 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: Rabbit Ig
• Specificity: This BCORL1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 69-98 amino acids from the N-terminal region of human BCORL1.
• Purity/Purification: This antibody is purified through a protein A column, followed by peptide affinity purification.
• Form/Format: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.
• Sequence Length: 1,785

• Applicable Applications for anti-BCORL1 antibody: Flow Cytometry (FC/FACS), ELISA (EIA), Western Blot (WB), Immunohistochemistry Paraffin (IHC-P)
• Application Notes: WB~~1:1000; IHC-P~~1:50~100; FC~~1:10~50
• Antigen Type: Synthetic Peptide
• Function: Transcriptional corepressor. May specifically inhibit gene expression when recruited to promoter regions by sequence-specific DNA- binding proteins such as BCL6. This repression may be mediated at least in part by histone deacetylase activities which can associate with this corepressor.
• Tissue Location: Detected in testis and prostate. Detected at lower levels in peripheral blood leukocytes and spleen
• Cellular Location: Nucleus.
• Antigen Source: HUMAN
• Antigen Region: 69-98
• Preparation and Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20 °C in small aliquots to prevent freeze-thaw cycles.

FCM (Flow Cytometry)

(BCORL1 Antibody (N-term) (AAA28773) flow cytometric analysis of HL-60 cells (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

IHC (Immunohistchemistry)

(BCORL1 antibody (N-term) (AAA28773) immunohistochemistry analysis in formalin fixed and paraffin embedded human prostate carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the BCORL1 antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.)

WB (Western Blot)

(BCORL1 Antibody (N-term) (AAA28773) western blot analysis in HL-60 cell line lysates (35ug/lane).This demonstrates the BCORL1 antibody detected the BCORL1 protein (arrow).)

WB (Western Blot)

(Anti-BCORL1 Antibody (N-term) at 1:500 dilution + Rat skeletal muscle lysate.Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 183 kDa Blocking/Dilution buffer: 5% NFDM/TBST.)

WB (Western Blot)

(Anti-BCORL1 Antibody (N-term) at 1:1000 dilution + Caco2 whole cell lysate.Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 183 kDa Blocking/Dilution buffer: 5% NFDM/TBST.)

WB (Western Blot)

(Anti-BCORL1 Antibody (N-term) at 1:500 dilution + Rat skeletal muscle lysate.Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 183 kDa Blocking/Dilution buffer: 5% NFDM/TBST.)

WB (Western Blot)

(Anti-BCORL1 Antibody (N-term) at 1:500 dilution + HL-60 whole cell lysate.Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 183 kDa Blocking/Dilution buffer: 5% NFDM/TBST.)

Related Product Information for anti-BCORL1 antibody

The protein encoded by this gene is a transcriptional
corepressor that is found tethered to promoter regions by
DNA-binding proteins. The encoded protein can interact with several
different class II histone deacetylases to repress transcription.
Two transcript variants encoding different isoforms have been found
for this gene.

References

Pagan, J.K., et al. J. Biol. Chem. 282(20):15248-15257(2007)
Lose, F., et al. Breast Cancer Res. 9 (4), R54 (2007) :

NCBI and Uniprot Product Information

• NCBI GI #: 296179431
• NCBI GeneID: 63035
• NCBI Accession #: NP_068765.3
• NCBI GenBank Nucleotide #: NM_021946.4
• UniProt Accession #: Q5H9F3; Q5H9F2; Q5H9F4; Q6ZVE0; Q8TEN3; Q9Y528; B5MDQ8
• NCBI Official Full Name: BCL-6 corepressor-like protein 1 1
• NCBI Official Synonym Full Names: BCL6 corepressor-like 1
• NCBI Official Symbol: BCORL1
• NCBI Official Synonym Symbols: BCoR-L1; CXorf10
• NCBI Protein Information: BCL-6 corepressor-like protein 1
• UniProt Protein Name: BCL-6 corepressor-like protein 1
• UniProt Gene Name: BCORL1
• UniProt Synonym Gene Names: BCoR-L1; BCoR-like protein 1
• UniProt Entry Name: BCORL_HUMAN