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IHC (Immunohistochemistry)
(Bcl-XL was detected in paraffin-embedded sections of human mammary cancer tissues using rabbit anti- Bcl-XL Antigen Affinity purified polyclonal antibody at 1ug/mL. The immunohistochemical section was developed using SABC method.)
Rabbit Bcl-XL Polyclonal Antibody | anti-BCL2L1 antibody
Anti-Bcl-XL Antibody
Gene Names
BCL2L1; BCLX; BCL2L; Bcl-X; PPP1R52; BCL-XL/S
Reactivity
Human, Mouse, Rat
Applications
Immunohistochemistry, Western Blot
Purity
Immunogen affinity purified.
Synonyms
Bcl-XL, Antibody; Anti-Bcl-XL Antibody; Bcl X; Bcl xL; BCL XL/S; Bcl xS; Bcl2-L-1; BCL2L; Bcl2l1; BCLX; BclXL; BclXs; PPP1R52; Q07817; Bcl-2-like protein 1; BCL2-like 1; anti-BCL2L1 antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Immunogen affinity purified.
Form/Format
Lyophilized
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Sequence Length
170
Applicable Applications for anti-BCL2L1 antibody
IHC (Immunohistochemistry), WB (Western Blot)
Notes
Tested Species: In-house tested species with positive results.
By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections.
Other applications have not been tested.
By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections.
Other applications have not been tested.
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Immunogen
E Coli-derived human Bcl-XL recombinant protein (Position: M1-T219). Human Bcl-XL shares 97.9% amino acid (aa) sequence identity with both mouse and rat Bcl-XL.
Preparation and Storage
Store at -20 degree C for one year. After reconstitution, at 4 degree C for one month. It can also be aliquotted and stored frozen at -20 degree C for a longer time. Avoid repeated freezing and thawing.
IHC (Immunohistochemistry)
(Bcl-XL was detected in paraffin-embedded sections of human mammary cancer tissues using rabbit anti- Bcl-XL Antigen Affinity purified polyclonal antibody at 1ug/mL. The immunohistochemical section was developed using SABC method.)
IHC (Immunohistochemistry)
(Bcl-XL was detected in paraffin-embedded sections of human mammary cancer tissues using rabbit anti- Bcl-XL Antigen Affinity purified polyclonal antibody at 1ug/mL. The immunohistochemical section was developed using SABC method.)
IHC (Immunohistochemisry)
(Bcl-XL was detected in paraffin-embedded sections of human lung cancer tissues using rabbit anti- Bcl-XL Antigen Affinity purified polyclonal antibody at 1ug/mL. The immunohistochemical section was developed using SABC method.)
IHC (Immunohistochemisry)
(Bcl-XL was detected in paraffin-embedded sections of human lung cancer tissues using rabbit anti- Bcl-XL Antigen Affinity purified polyclonal antibody at 1ug/mL. The immunohistochemical section was developed using SABC method.)
IHC (Immunohiostchemistry)
(Bcl-XL was detected in paraffin-embedded sections of human tonsil tissues using rabbit anti- Bcl-XL Antigen Affinity purified polyclonal antibody at 1ug/mL. The immunohistochemical section was developed using SABC method.)
IHC (Immunohiostchemistry)
(Bcl-XL was detected in paraffin-embedded sections of human tonsil tissues using rabbit anti- Bcl-XL Antigen Affinity purified polyclonal antibody at 1ug/mL. The immunohistochemical section was developed using SABC method.)
WB (Western Blot)
(Western blot analysis of Bcl-XL expression in rat thymus extract (lane 1), NIH3T3 whole cell lysates lane 2), HEPA1-6 whole cell lysates (lane 3), SGC7901 whole cell lysates (lane 4) and HEPG2 whole cell lysates (lane 5). Bcl-XL at 30KD was detected using rabbit anti- Bcl-XL Antigen Affinity purified polyclonal antibody at 0.5ug/mL. The blot was developed using chemiluminescence (ECL) method.)
WB (Western Blot)
(Western blot analysis of Bcl-XL expression in rat thymus extract (lane 1), NIH3T3 whole cell lysates lane 2), HEPA1-6 whole cell lysates (lane 3), SGC7901 whole cell lysates (lane 4) and HEPG2 whole cell lysates (lane 5). Bcl-XL at 30KD was detected using rabbit anti- Bcl-XL Antigen Affinity purified polyclonal antibody at 0.5ug/mL. The blot was developed using chemiluminescence (ECL) method.)
Related Product Information for anti-BCL2L1 antibody
Rabbit IgG polyclonal antibody for Bcl-2-like protein 1(BCL2L1) detection.
Background: Bcl-2-like protein 1, also known as Bcl-XL, is a protein that in humans is encoded by the BCL2L1 gene. The protein encoded by this gene belongs to the BCL-2 protein family. BCL-2 family members form hetero- or homodimers and act as anti- or pro-apoptotic regulators that are involved in a wide variety of cellular activities. The proteins encoded by this gene are located at the outer mitochondrial membrane, and have been shown to regulate outer mitochondrial membrane channel (VDAC) opening. VDAC regulates mitochondrial membrane potential, and thus controls the production of reactive oxygen species and release of cytochrome C by mitochondria, both of which are the potent inducers of cell apoptosis. Alternative splicing results in multiple transcript variants encoding two different isoforms. The longer isoform (Bcl-xL) acts as an apoptotic inhibitor and the shorter form (Bcl-xS) acts as an apoptotic activator.
Background: Bcl-2-like protein 1, also known as Bcl-XL, is a protein that in humans is encoded by the BCL2L1 gene. The protein encoded by this gene belongs to the BCL-2 protein family. BCL-2 family members form hetero- or homodimers and act as anti- or pro-apoptotic regulators that are involved in a wide variety of cellular activities. The proteins encoded by this gene are located at the outer mitochondrial membrane, and have been shown to regulate outer mitochondrial membrane channel (VDAC) opening. VDAC regulates mitochondrial membrane potential, and thus controls the production of reactive oxygen species and release of cytochrome C by mitochondria, both of which are the potent inducers of cell apoptosis. Alternative splicing results in multiple transcript variants encoding two different isoforms. The longer isoform (Bcl-xL) acts as an apoptotic inhibitor and the shorter form (Bcl-xS) acts as an apoptotic activator.
References
1. "Entrez Gene: BCL2L1 BCL2-like 1".
2. Boise LH, González-García M, Postema CE, Ding L, Lindsten T, Turka LA, Mao X, Nuñez G, Thompson CB (Aug 1993). "bcl-x, a bcl-2-related gene that functions as a dominant regulator of apoptotic cell death". Cell 74(4): 597-608.
2. Boise LH, González-García M, Postema CE, Ding L, Lindsten T, Turka LA, Mao X, Nuñez G, Thompson CB (Aug 1993). "bcl-x, a bcl-2-related gene that functions as a dominant regulator of apoptotic cell death". Cell 74(4): 597-608.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
25,290 Da
NCBI Official Full Name
bcl-2-like protein 1 isoform Bcl-X(S)
NCBI Official Synonym Full Names
BCL2 like 1
NCBI Official Symbol
BCL2L1
NCBI Official Synonym Symbols
BCLX; BCL2L; Bcl-X; PPP1R52; BCL-XL/S
NCBI Protein Information
bcl-2-like protein 1
UniProt Protein Name
Bcl-2-like protein 1
UniProt Gene Name
BCL2L1
UniProt Synonym Gene Names
BCL2L; BCLX; Bcl2-L-1
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Product Notes
The BCL2L1 bcl2l1 (Catalog #AAA46711) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-Bcl-XL Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's Bcl-XL can be used in a range of immunoassay formats including, but not limited to, IHC (Immunohistochemistry), WB (Western Blot). Researchers should empirically determine the suitability of the BCL2L1 bcl2l1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Bcl-XL, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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