Rabbit anti-Human Apolipoprotein A-I/APOA1 Polyclonal Antibody | anti-APOA1 antibody

Anti-Apolipoprotein A-I/APOA1 Antibody, Rabbit Polyclonal

Cat. Num. AAA257519

• Reactivity: Human
• Applications: Immunoprecipitation, Immunohistochemistry, ELISA, Western Blot
• Purity: Protein A & Antigen Affinity
• Synonyms: Apolipoprotein A-I/APOA1, Antibody; Anti-Apolipoprotein A-I/APOA1 Antibody, Rabbit Polyclonal; APOA1/ApoAI Antibody, Rabbit PAb, Antigen Affinity Purified; Anti-Apolipoprotein A-I Antibody; apolipoprotein A-I; anti-APOA1 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Specificity: Human APOA1/ApoAI
• Purity/Purification: Protein A & Antigen Affinity
• Form/Format: Liquid. 0.2um filtered solution in PBS

• Applicable Applications for anti-APOA1 antibody: IP (Immunoprecipitation), IHC (Immunohistochemistry), ELISA, WB (Western Blot)
• Immunogen: Recombinant Human APOA1/ApoAI protein
• Conjugation: Unconjugated
• Preparation: Produced in rabbits immunized with purified, recombinant Human APOA1/ApoAI (rh APOA1/ApoAI; P02647; Asp25-Gln267).APOA1/ApoAI specific IgG was purified by Human APOA1/ApoAI affinity chromatography.
• Preparation and Storage: This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Preservative-Free. Avoid repeated freeze-thaw cycles.; This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

WB (Western Blot)

(Anti-APOA1 rabbit polyclonal antibody at 1:500 dilutionLane A: HepG2 Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.Developed using the Odyssey technique.Performed under reducing conditions.Predicted band size:31 kDaObserved band size:27 kDa(We are unsure as to the identity of these extra bands.))

IP (Immunoprecipitation)

(APOA1 was immunoprecipitated using:Lane A:0.5 mg HepG2 Whole Cell Lysate2 uL anti-APOA1 rabbit polyclonal antibody and 15 ul of 50 % Protein G agarose.Primary antibody:Anti-APOA1 rabbit polyclonal antibody,at 1:200 dilutionSecondary antibody:Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilutionDeveloped using the odssey technique.Performed under reducing conditions.Predicted band size: 27 kDaObserved band size: 27 kDa)

IHC (Immunohistochemistry)

(Immunochemical staining of human APOA1 in human cirrhosis with rabbit polyclonal antibody (1:10000, formalin-fixed paraffin embedded sections).)

Related Product Information for anti-APOA1 antibody

Background: Apolipoprotein A1 (APOA1) is a member of the apolipoprotein family whose members are proteins bind with lipids and form lipoproteins to translate these oil-soluble lipids such as fat and cholesterol through lymphatic and circulatory system. APOA1 is the main component of high density lipoprotein (HDL) in plasma and is involved in the esterification of cholesterol as a cofactor of lecithin-cholesterol acyltransferase (LCAT) which is responsible for the formation of most plasma cholesteryl esters, and thus play a major role in cholesterol efflux from peripheral cells. As a major component of the HDL complex, APOA1 helps to clear cholesterol from arteries. APOA1 is also characterized as a prostacyclin stabilizing factor, and thus may have an anticlotting effect. Defects in encoding gene may result in HDL deficiencies, including Tangier disease, and with systemic non-neuropathic amyloidosis. Men carrying a mutation may develop premature coronary artery disease.

References

Toptas B, et al. (2011) Comparison of lipid profiles with APOA1 MspI polymorphism in obese children with hyperlipidemia. In Vivo. 25(3): 425-30.Haase CL, et al. (2011) Mutation in APOA1 predicts increased risk of ischaemic heart disease and total mortality without low HDL cholesterol levels. J Intern Med. 270(2): 136-46.Wu Z, et al. (2011) The low resolution structure of ApoA1 in spherical high density lipoprotein revealed small angle neutron scattering. J Biol Chem. 286(14): 12495-508.