Standard Curve (Sample)
Mouse Tyrosine-protein kinase BTK ELISA Kit | Btk elisa kit
Mouse Tyrosine-protein kinase BTK ELISA Kit
Gene Names
Btk; xid; AI528679
Reactivity
Mouse
Synonyms
Tyrosine-protein kinase BTK; N/A; Mouse Tyrosine-protein kinase BTK ELISA Kit; Agammaglobulinaemia tyrosine kinase; ATK; B-cell progenitor kinase; BPK; Bruton tyrosine kinase; Kinase EMB; Btk; Bpk; 2.7.10.2; Btk elisa kit
Reactivity
Mouse
Specificity
This assay recognizes recombinant and natural mouse Tyrosine-protein kinase BTK. No significant cross-reactivity or interference was observed.
Sequence Length
659
Assay Type
Sandwich
Detection Range
0.156-10 ng/mL
Sensitivity
0.092 ng/mL
Intra-Assay CV
<=8.8%
Inter-Assay CV
<=11.0%
Recovery
99%
Preparation and Storage
For long term storage, please store the entire kit at -20 degree C.
Standard Curve (Sample)
Standard Curve (Sample)
Related Product Information for Btk elisa kit
Intended Uses: This immunoassay kit allows for the in vitro quantitative determination of mouse Tyrosine-protein kinase BTK concentrations in serum, Plasma, tissue homogenates and Cell culture supernates and Other biological fluids.
Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to Tyrosine-protein kinase BTK. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for Tyrosine-protein kinase BTK and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Tyrosine-protein kinase BTK, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of Tyrosine-protein kinase BTK in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to Tyrosine-protein kinase BTK. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for Tyrosine-protein kinase BTK and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Tyrosine-protein kinase BTK, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of Tyrosine-protein kinase BTK in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
76,437 Da
NCBI Official Full Name
tyrosine-protein kinase BTK
NCBI Official Synonym Full Names
Bruton agammaglobulinemia tyrosine kinase
NCBI Official Symbol
Btk
NCBI Official Synonym Symbols
xid; AI528679
NCBI Protein Information
tyrosine-protein kinase BTK
UniProt Protein Name
Tyrosine-protein kinase BTK
UniProt Gene Name
Btk
UniProt Synonym Gene Names
Bpk; ATK; BPK
UniProt Entry Name
BTK_MOUSE
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Product Notes
The Mouse Btk btk (Catalog #AAA57294) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA57294 ELISA Kit recognizes Mouse Btk. It is sometimes possible for the material contained within the vial of "Tyrosine-protein kinase BTK, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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