Standard Curve (Sample)
Mouse SLIT2 ELISA Kit | SLIT2 elisa kit
Mouse SLIT2 PicoKine ELISA Kit
Gene Names
Slit2; Slil3; Drad-1; slit-2; mKIAA4141; b2b1200.1Clo; E030015M03Rik; E130320P19Rik
Reactivity
Mouse
Synonyms
SLIT2; N/A; Mouse SLIT2 PicoKine ELISA Kit; Mouse SLIT2; slit guidance ligand 2; Slit homolog 2 protein; Slit-2; Slit homolog 2 protein N-product; Slit homolog 2 protein C-product; Slit2; SLIT2 elisa kit
Reactivity
Mouse
Specificity
Natural and recombinant Mouse Slit2
Sequence Length
1521
Samples
Cell culture supernates, serum and plasma (heparin, EDTA)
Detection Range
46.9pg/ml-3000pg/ml
Sensitivity
<10pg/ml
Intra-assay Precision
Intra-Assay Precision (Precision within an assay): Three samples of known concentration were tested on one plate to assess intra-assay precision.
Inter-assay Precision
Inter-Assay Precision (Precision across assays):Three samples of known concentration were tested in separate assays to assess inter-assay precision.
Preparation and Storage
Store at 4 degree C for 6 months. Store at -20 degree C for 12 months. Avoid multiple freeze-thaw cycles.
Standard Curve (Sample)
Standard Curve (Sample)
Related Product Information for SLIT2 elisa kit
Principle of the Assay: The Picokine™ Mouse Slit2 Pre-Coated ELISA (Enzyme-Linked Immunosorbent Assay) kit is a solid phase immunoassay specially designed to measure Mouse Slit2 with a 96-well strip plate that is pre-coated with antibody specific for Slit2. The detection antibody is a biotinylated antibody specific for Slit2. The capture antibody is a monoclonal antibody from rat, the detection antibody is a biotinylated polyclonal antibody from goat. The kit contains recombinant Mouse Slit2 with immunogen: Expression system for standard: NSO, Immunogen sequence: Q26-Q900. The kit is analytically validated with ready to use reagents. To measure Mouse Slit2, add standards and samples to the wells, then add the biotinylated detection antibody. Wash the wells with PBS or TBS buffer, and add Avidin-Biotin-Peroxidase Complex (ABC-HRP). Wash away the unbounded ABC-HRP with PBS or TBS buffer and add TMB. TMB is substrate to HRP and will be catalyzed to produce a blue color product, which changes into yellow after adding acidic stop solution. The density of the yellow product is linearly proportional to Mouse Slit2 in the sample. Read the density of the yellow product in each well using a plate reader, and benchmark the sample wells' readings against the standard curve to determine the concentration of Mouse Slit2 in the sample.!!Background/Introduction: Slit homolog 2 protein is a protein that in humans is encoded by the SLIT2 gene. It is mapped to 5; 5 B3. The protein encoded by this gene is a member of the Slit family of secreted glycoproteins, which function as ligands for the Robo family of immunoglobulin receptors. Slit proteins play highly conserved roles in axon guidance and neuronal migration and may also have functions during other cell migration processes including leukocyte migration. In mammals, members of the slit family are characterized by an N-terminal signal peptide, four leucine-rich repeats, nine epidermal growth factor repeats, and a C-terminal cysteine knot. Mice deficient for this gene exhibit abnormal axonal projections in the embryonic forebrain and develop supernumerary uretic buds that maintain improper connections to the nephric duct. Alternative splicing results in multiple transcript variants.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
slit homolog 2 protein isoform c
NCBI Official Synonym Full Names
slit guidance ligand 2
NCBI Official Symbol
Slit2
NCBI Official Synonym Symbols
Slil3; Drad-1; slit-2; mKIAA4141; b2b1200.1Clo; E030015M03Rik; E130320P19Rik
NCBI Protein Information
slit homolog 2 protein
UniProt Protein Name
Slit homolog 2 protein
UniProt Gene Name
Slit2
UniProt Synonym Gene Names
Slit-2
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Product Notes
The Mouse SLIT2 slit2 (Catalog #AAA125201) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA125201 ELISA Kit recognizes Mouse SLIT2. It is sometimes possible for the material contained within the vial of "SLIT2, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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