FCM (Flow Cytometry)
(Flow cytometric analysis of SH-SY5Y cells with S100 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.)
Rabbit anti-Human, Mouse S100 Monoclonal Antibody | anti-S100 antibody
S100 Antibody
IHC: 1:50-1:200
IP: 1:10-1:50
FC/FACS: 1:50-1:100
FCM (Flow Cytometry)
(Flow cytometric analysis of SH-SY5Y cells with S100 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.)
FCM (Flow Cytometry)
(Flow cytometric analysis of SH-SY5Y cells with S100 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.)
IHC (Immunohistochemistry)
(Immunohistochemical analysis of paraffin-embedded mouse spinal cord tissue using anti-S100 antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry)
(Immunohistochemical analysis of paraffin-embedded mouse spinal cord tissue using anti-S100 antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry)
(Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti- S100 antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry)
(Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti- S100 antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry)
(Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti- S100 antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry)
(Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti- S100 antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry)
(Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti- S100 antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry)
(Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti- S100 antibody. Counter stained with hematoxylin.)
WB (Western Blot)
(Western blot analysis of S100 on mouse heart cells lysates using anti-S100 antibody at 1/500 dilution.)
WB (Western Blot)
(Western blot analysis of S100 on mouse heart cells lysates using anti-S100 antibody at 1/500 dilution.)
NCBI and Uniprot Product Information
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Product Notes
The S100 s100a1 (Catalog #AAA30331) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The S100 Antibody reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's S100 can be used in a range of immunoassay formats including, but not limited to, WB (Western Blot), IP (Immunoprecipitation), IHC (Immunohistochemistry), FCM/FACS (Flow Cytometry). WB: 1:500-1:2000 IHC: 1:50-1:200 IP: 1:10-1:50 FC/FACS: 1:50-1:100. Researchers should empirically determine the suitability of the S100 s100a1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "S100, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Frequently Asked Questions
What cell types or tissues express S100 proteins that this antibody can detect?
S100 proteins are expressed in neural crest-derived cells, including Schwann cells, melanocytes, dendritic cells, glial cells, and certain immune cell subsets. This antibody specifically detects S100 family members (particularly S100A and S100B), which are abundant in nervous tissue, skin, and tumors derived from these cell lineages.
Can an S100 monoclonal antibody be used for immunohistochemistry in tumor diagnosis?
Yes, S100 IHC is a clinical standard for melanoma diagnosis and differential diagnosis of neural-derived tumors. This monoclonal antibody enables reliable identification of melanomas (96-99% sensitivity) in tissue sections, distinguishing melanoma from other malignancies and assisting in tumor staging and classification.
What’s the difference between S100A and S100B detection with this antibody?
S100A and S100B represent different S100 family members with distinct tissue distributions and functions. Some S100 antibodies show specificity for individual family members, while broader-spectrum antibodies detect multiple S100 proteins. The specific antibody specificity determines whether results reflect total S100 or particular isoforms.
Is S100 expression associated with melanoma or other cancers?
S100 is strongly associated with melanoma but shows variable expression in other cancers, including glioma, peripheral nerve sheath tumors, and some sarcomas. S100 elevation in melanoma correlates with tumor burden and disease progression; however, S100 also appears in some benign neural tumors, limiting its diagnostic specificity.
Can the S100 antibody be used for immunofluorescence or Western blot applications?
Yes, S100 antibodies are validated for immunofluorescence (enabling visualization of S100+ tumor cells and Schwann cells) and Western blotting (quantifying S100 protein levels). Serum S100B measurement via ELISA also predicts melanoma metastasis and disease relapse, providing prognostic utility beyond histopathology.
Is S100 commonly used as a marker for neural crest or Schwann cells?
Yes, S100 is a classic marker for neural crest-derived cells, particularly Schwann cells in peripheral nerves and satellite glial cells. S100 expression is used to identify nerve structures in tissue sections and Schwann cell populations in cell biology studies, facilitating studies of neural development and peripheral nerve pathology.
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