WB (Western Blot)
(RUNX1 monoclonal antibody Western Blot analysis of RUNX1 expression in Hela NE.)
Mouse anti-Human, Mouse RUNX1 Monoclonal Antibody | anti-RUNX1 antibody
RUNX1 (Runt-related Transcription Factor 1, Acute Myeloid Leukemia 1 Protein, Core-binding Factor Subunit alpha-2, CBF-alpha-2, Oncogene AML-1, Polyomavirus Enhancer-binding Protein 2 alpha B Subunit, PEA2-alpha B, PEBP2-alpha B, SL3-3 Enhancer Factor 1 a
Applications are based on unconjugated antibody.
WB (Western Blot)
(RUNX1 monoclonal antibody Western Blot analysis of RUNX1 expression in Hela NE.)
WB (Western Blot)
(RUNX1 monoclonal antibody Western Blot analysis of RUNX1 expression in Hela NE.)
Application Data
(Detection limit for recombinant GST tagged RUNX1 is ~1ng/ml as a capture antibody.)
Application Data
(Detection limit for recombinant GST tagged RUNX1 is ~1ng/ml as a capture antibody.)
IF (Immunofluorescence)
(Immunofluorescence of monoclonal antibody to RUNX1 on HeLa cell. [antibody concentration 10ug/ml].)
IF (Immunofluorescence)
(Immunofluorescence of monoclonal antibody to RUNX1 on HeLa cell. [antibody concentration 10ug/ml].)
IHC (Immunohistochemistry)
(Immunoperoxidase of monoclonal antibody to RUNX1 on formalin-fixed paraffin-embedded human placenta. [antibody concentration 3ug/ml].)
IHC (Immunohistochemistry)
(Immunoperoxidase of monoclonal antibody to RUNX1 on formalin-fixed paraffin-embedded human placenta. [antibody concentration 3ug/ml].)
WB (Western Blot)
(RUNX1 monoclonal antibody Western Blot analysis of RUNX1 expression in NIH/3T3.)
WB (Western Blot)
(RUNX1 monoclonal antibody Western Blot analysis of RUNX1 expression in NIH/3T3.)
WB (Western Blot)
(Western Blot detection against Immunogen (36.85kD).)
WB (Western Blot)
(Western Blot detection against Immunogen (36.85kD).)
NCBI and Uniprot Product Information
Similar Products
Product Notes
The RUNX1 runx1 (Catalog #AAA24940) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The RUNX1 (Runt-related Transcription Factor 1, Acute Myeloid Leukemia 1 Protein, Core-binding Factor Subunit alpha-2, CBF-alpha-2, Oncogene AML-1, Polyomavirus Enhancer-binding Protein 2 alpha B Subunit, PEA2-alpha B, PEBP2-alpha B, SL3-3 Enhancer Factor 1 a reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's RUNX1 can be used in a range of immunoassay formats including, but not limited to, ELISA, IF (Immunofluorescence), IHC (Immunohistochemistry), WB (Western Blot). IF: 10ug/ml Applications are based on unconjugated antibody. Researchers should empirically determine the suitability of the RUNX1 runx1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "RUNX1, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Frequently Asked Questions
What cell types or tissues express RUNX1 that this antibody can detect?
RUNX1 is preferentially expressed in hematopoietic tissues, including bone marrow, thymus, and lymph nodes. This monoclonal antibody detects RUNX1 in hematopoietic stem cells, progenitor cells, and mature blood lineages (B cells, T cells, myeloid cells, megakaryocytes, erythrocytes). Expression is highest during early hematopoietic differentiation and remains detectable across all myeloid and lymphoid developmental stages.
Can a mouse anti-human RUNX1 monoclonal antibody be used for flow cytometry?
While this clone 4E7 is validated for IF, IHC, WB, and ELISA, flow cytometry use requires specific optimization. For FCM/FACS applications, we recommend validating against your specific cell population and equipment, or consulting technical support for conjugated versions or alternative clones optimized for flow cytometry analysis.
Is RUNX1 expression associated with hematopoietic stem cells or leukemia research?
Yes. RUNX1 is critical for hematopoietic stem cell maintenance and differentiation. Chromosomal translocations involving RUNX1 - particularly t(8;21)- are hallmark features of acute myeloid leukemia (AML) subtype M2, making this antibody essential for studying hematopoietic development and core-binding factor malignancies.
Can this RUNX1 antibody be used for immunofluorescence or Western blot applications?
Yes, clone 4E7 is specifically validated for both IF and WB applications. For IF, it provides an excellent nuclear signal in fixed cells and tissues reflecting RUNX1's transcription factor localization. For WB, use 1-5 μg/mL as the starting concentration; expect a band at approximately 53 kDa corresponding to full-length RUNX1.
How does RUNX1 differ from other transcription factors in blood cell development?
RUNX1 uniquely functions as a master regulator through its runt homology domain (RHD), which directly binds DNA at enhancer elements. Unlike other transcription factors, RUNX1 forms a heterodimeric complex with CBFβ that increases DNA-binding affinity. This core-binding factor mechanism controls genes crucial for hematopoietic stem cell maintenance and lineage commitment.
Is this antibody suitable for detecting RUNX1 in fixed tissue samples?
Yes. This monoclonal antibody is well-suited for immunohistochemistry (IHC) in formalin-fixed, paraffin-embedded (FFPE) sections. Nuclear localization of RUNX1 provides strong, specific staining with minimal background, making it ideal for diagnostic pathology and research tissue applications.
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