IF (Immunofluorescence)
(Immunofluorescence Analysis of T98G cells labeling Pgp9.5 with Pgp9.5 / UchL1 Mouse Monoclonal Antibody (31A3) followed by Goat anti-Mouse IgG-CF488 (Green). The nuclear counterstain is Nucspot (Red))
Mouse PGP9.5 / UchL1 Monoclonal Antibody | anti-UCHL1 antibody
PGP9.5 / UchL1 (pan-Neuronal Marker) Mouse Monoclonal Antibody
Immunohistochemistry (Formalin-fixed) (0.5-1ug/ml for 30 minutes at RT) (Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes)
IF (Immunofluorescence)
(Immunofluorescence Analysis of T98G cells labeling Pgp9.5 with Pgp9.5 / UchL1 Mouse Monoclonal Antibody (31A3) followed by Goat anti-Mouse IgG-CF488 (Green). The nuclear counterstain is Nucspot (Red))
IF (Immunofluorescence)
(Immunofluorescence Analysis of T98G cells labeling Pgp9.5 with Pgp9.5 / UchL1 Mouse Monoclonal Antibody (31A3) followed by Goat anti-Mouse IgG-CF488 (Green). The nuclear counterstain is Nucspot (Red))
FCM (Flow Cytometry)
(Flow Cytometric Analysis of T98G cells using Pgp9.5 / UchL1 Mouse Monoclonal Antibody (31A3) followed by Goat anti-Mouse IgG-CF488 (Blue); Isotype Control (Red).)
FCM (Flow Cytometry)
(Flow Cytometric Analysis of T98G cells using Pgp9.5 / UchL1 Mouse Monoclonal Antibody (31A3) followed by Goat anti-Mouse IgG-CF488 (Blue); Isotype Control (Red).)
WB (Western Blot)
(Western Blot Analysis of human brain tissue lysate using Pgp9.5 / UchL1 Mouse Monoclonal Antibody (31A3).)
WB (Western Blot)
(Western Blot Analysis of human brain tissue lysate using Pgp9.5 / UchL1 Mouse Monoclonal Antibody (31A3).)
WB (Western Blot)
(Western Blot of UchL1 (PGP9.5) in 1) human, 2) mouse and 3) rat brain Lysate using UchL1 Monoclonal Antibody (31A3).)
WB (Western Blot)
(Western Blot of UchL1 (PGP9.5) in 1) human, 2) mouse and 3) rat brain Lysate using UchL1 Monoclonal Antibody (31A3).)
IHC (Immunohistochemistry)
(Formalin-fixed, paraffin-embedded human Cerebellum stained with Pgp9.5 / UchL1 Monoclonal Antibody (31A3).)
IHC (Immunohistochemistry)
(Formalin-fixed, paraffin-embedded human Cerebellum stained with Pgp9.5 / UchL1 Monoclonal Antibody (31A3).)
NCBI and Uniprot Product Information
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Product Notes
The UCHL1 uchl1 (Catalog #AAA13809) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The PGP9.5 / UchL1 (pan-Neuronal Marker) Mouse Monoclonal Antibody reacts with Human, Mouse, Rat, Cow, Pig, Dog and may cross-react with other species as described in the data sheet. AAA Biotech's PGP9.5 / UchL1 can be used in a range of immunoassay formats including, but not limited to, WB (Western Blot), IHC (Immunohistochemistry). Western Blot (0.5-1ug/ml) Immunohistochemistry (Formalin-fixed) (0.5-1ug/ml for 30 minutes at RT) (Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes). Researchers should empirically determine the suitability of the UCHL1 uchl1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "PGP9.5 / UchL1, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Frequently Asked Questions
What is PGP9.5 (UCHL1?
PGP9.5 (UCHL1) is a protein in nerve cells that aids protein recycling. Because it is highly present in nerves, scientists often use it as a marker to identify neurons and nerve fibers in research studies.
Is PGP9.5 a reliable marker for neurons or nerve fibers?
Yes, PGP9.5 is a reliable marker for neurons and nerve fibers—strongly expressed in nerve cells. Researchers use it to study the nervous system structure and nerve patterns.
Can a PGP9.5 antibody be used for immunohistochemistry or immunofluorescence?
Yes, the PGP9.5 antibody is used in immunohistochemistry or immunofluorescence to stain and locate nerve cells in tissues under a microscope for nerve structure research.
Is PGP9.5 expression specific to neural tissue in mice?
PGP9.5 is mainly expressed in neural tissue in mice, including neurons and nerve fibers. However, small amounts may appear in some neuroendocrine cells. Overall, it remains a strong and reliable neural marker in mouse research.
How does PGP9.5 staining help in studying neurodegeneration or nerve injury?
PGP9.5 staining helps show the presence and condition of nerve fibers. In nerve damage or neurodegeneration, PGP9.5 staining decreases or changes. This helps researchers track nerve loss, regeneration, or structural shifts in disease or injury.
Can this antibody detect PGP9.5 in both central and peripheral nervous system tissues?
Yes, this antibody can detect PGP9.5 in both the central nervous system and the peripheral nervous system. Since PGP9.5 is present in neurons throughout the body, the antibody is useful for studying many nerve tissues.
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