Application Data
(Overlay histogram showing Hela cells stained with AAA18857 (red line). The cells were fixed with 70% Ethylalcohol (18h) and then permeabilized with 0.3% Triton X-100 for 2 min. The cells were then incubated in 1x PBS /10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (10ug/1x10^6cells) for 1 h at 4 degree C. The secondary antibody used was FITC goat anti-mouse IgG (H+L) at 1/200 dilution for 1 h at 4 degree C. Isotype control antibody (green line) was mouse IgG1 (10ug/1x10^6cells) used under the same conditions. Acquisition of >10,000 events was performed.)
Mouse Nestin Monoclonal Antibody | anti-NES antibody
Mouse Anti-Nestin monoclonal Antibody
Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
Application Data
(Overlay histogram showing Hela cells stained with AAA18857 (red line). The cells were fixed with 70% Ethylalcohol (18h) and then permeabilized with 0.3% Triton X-100 for 2 min. The cells were then incubated in 1x PBS /10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (10ug/1x10^6cells) for 1 h at 4 degree C. The secondary antibody used was FITC goat anti-mouse IgG (H+L) at 1/200 dilution for 1 h at 4 degree C. Isotype control antibody (green line) was mouse IgG1 (10ug/1x10^6cells) used under the same conditions. Acquisition of >10,000 events was performed.)
Application Data
(Overlay histogram showing Hela cells stained with AAA18857 (red line). The cells were fixed with 70% Ethylalcohol (18h) and then permeabilized with 0.3% Triton X-100 for 2 min. The cells were then incubated in 1x PBS /10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (10ug/1x10^6cells) for 1 h at 4 degree C. The secondary antibody used was FITC goat anti-mouse IgG (H+L) at 1/200 dilution for 1 h at 4 degree C. Isotype control antibody (green line) was mouse IgG1 (10ug/1x10^6cells) used under the same conditions. Acquisition of >10,000 events was performed.)
Application Data
(Overlay histogram showing U251 cells stained with AAA18857 (red line). The cells were fixed with 70% Ethylalcohol (18h) and then permeabilized with 0.3% Triton X-100 for 2 min. The cells were then incubated in 1x PBS /10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (10ug/1x10^6cells) for 1 h at 4 degree C. The secondary antibody used was FITC goat anti-mouse IgG (H+L) at 1/200 dilution for 1 h at 4 degree C. Isotype control antibody (green line) was mouse IgG1 (10ug/1x10^6cells) used under the same conditions. Acquisition of >10,000 events was performed.)
Application Data
(Overlay histogram showing U251 cells stained with AAA18857 (red line). The cells were fixed with 70% Ethylalcohol (18h) and then permeabilized with 0.3% Triton X-100 for 2 min. The cells were then incubated in 1x PBS /10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (10ug/1x10^6cells) for 1 h at 4 degree C. The secondary antibody used was FITC goat anti-mouse IgG (H+L) at 1/200 dilution for 1 h at 4 degree C. Isotype control antibody (green line) was mouse IgG1 (10ug/1x10^6cells) used under the same conditions. Acquisition of >10,000 events was performed.)
IF (Immunofluorescence)
(Immunofluorescent analysis of U251 cells using AAA18857 at a dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Mouse IgG(H+L))
IF (Immunofluorescence)
(Immunofluorescent analysis of U251 cells using AAA18857 at a dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Mouse IgG(H+L))
IF (Immunofluorescence)
(Immunofluorescent analysis of PC-3 cells using AAA18857 at a dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Mouse IgG(H+L))
IF (Immunofluorescence)
(Immunofluorescent analysis of PC-3 cells using AAA18857 at a dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Mouse IgG(H+L))
IF (Immunofluorescence)
(Immunofluorescent analysis of Hela cells using AAA18857 at a dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Mouse IgG(H+L))
IF (Immunofluorescence)
(Immunofluorescent analysis of Hela cells using AAA18857 at a dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Mouse IgG(H+L))
IHC (Immunohistochemistry)
(Immunohistochemistry of paraffin-embedded human melanoma using AAA18857 at dilution of 1:100.)
IHC (Immunohistochemistry)
(Immunohistochemistry of paraffin-embedded human melanoma using AAA18857 at dilution of 1:100.)
IHC (Immunohistochemistry)
(Immunohistochemistry of paraffin-embedded human kidney tissue using AAA18857 at dilution of 1:100.)
IHC (Immunohistochemistry)
(Immunohistochemistry of paraffin-embedded human kidney tissue using AAA18857 at dilution of 1:100.)
IHC (Immunohistochemistry)
(Immunohistochemistry of paraffin-embedded human tonsil tissue using AAA18857 at dilution of 1:100.)
IHC (Immunohistochemistry)
(Immunohistochemistry of paraffin-embedded human tonsil tissue using AAA18857 at dilution of 1:100.)
WB (Western Blot)
(Western Blot Positive WB detected in: U251 whole cell lysate, SH-Sy5y whole cell lysate All lanes: NES antibody at 3 ug/mlPredicted band size: 260 kDa Observed band size: 260 kDa )
WB (Western Blot)
(Western Blot Positive WB detected in: U251 whole cell lysate, SH-Sy5y whole cell lysate All lanes: NES antibody at 3 ug/mlPredicted band size: 260 kDa Observed band size: 260 kDa )
NCBI and Uniprot Product Information
Similar Products
Product Notes
The NES nes (Catalog #AAA18857) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's Nestin can be used in a range of immunoassay formats including, but not limited to, ELISA, WB (Western Blot), IHC (Immunohistochemistry), IF (Immunofluorescence), FCM/FACS (Flow Cytometry). Researchers should empirically determine the suitability of the NES nes for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Nestin, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Frequently Asked Questions
Is this Nestin antibody suitable for neural stem cell detection?
Yes, Nestin antibodies are well-suited for neural stem cell detection. Nestin is a reliable neural stem cell marker that shows co-expression with other NSC markers like GFAP. This monoclonal antibody effectively identifies neural progenitor cells and stem cells in various tissue preparations and cell cultures.
Which applications does it work best for (IF, IHC, WB)?
Nestin antibodies perform effectively across multiple applications. Immunofluorescence (IF) typically uses dilutions of 1:50-1:100, immunohistochemistry (IHC) employs 1:100-1:200 dilutions, and Western blot uses 1:3000 dilution with approximately 180 kDa molecular weight detection. All three methods are well-validated for Nestin detection.
What species does the Nestin antibody cross-react with?
Nestin antibodies commonly cross-react with human, mouse, and rat species, though reactivity varies. Some antibodies show species-specific limitations due to low sequence conservation across species boundaries - mouse and human Nestin share only 62% overall protein identity, requiring careful clone selection for desired species compatibility.
What dilution is recommended for staining neural tissues?
Recommended dilutions for neural tissue staining vary by application: immunohistochemistry typically uses 1:100-1:200 dilution, immunofluorescence uses 1:50-1:100, and Western blot employs 1:3000 for cellular lysates. Specific optimization depends on tissue type, fixation method, and detection system employed in your protocol.
Can it be used for stem cell differentiation studies?
Yes, Nestin antibodies are valuable for differentiation studies. During neural differentiation, Nestin expression decreases while neuronal markers (NeuN+) increase, and astrocytic markers (GFAP+) remain elevated in astrocyte differentiation. This dynamic expression pattern enables tracking of neural stem cell differentiation trajectories into specific lineages.
Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.