FCM (Flow Cytometry)
(Flow cytometric analysis of Human GOLM1(GP73) expression on HeLa cells. The cells were treated according to manufacturer's manual (BD Pharmingen'), stained with purified anti-Human GOLM1(GP73), then a FITC-conjugated second step antibody. The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of intact cells.)
Rabbit anti-Human GOLPH2/GOLM1 Monoclonal Antibody | anti-GOLM1 antibody
Recombinant Anti-GOLPH2/GOLM1 Antibody, Rabbit Monoclonal
Gene Names
GOLM1; GP73; HEL46; GOLPH2; C9orf155; PSEC0257; bA379P1.3
Reactivity
Human
Applications
ELISA, Flow Cytometry
Purity
Protein A
Synonyms
GOLPH2/GOLM1, Antibody; Recombinant Anti-GOLPH2/GOLM1 Antibody, Rabbit Monoclonal; GOLM1 Antibody, Rabbit MAb; golgi membrane protein 1; Anti-bA379P1.3 Antibody; Anti-C9orf155 Antibody; Anti-GOLPH2 Antibody; Anti-GP73 Antibody; Anti-HEL46 Antibody; Anti-PSEC0257 Antibody; anti-GOLM1 antibody
Host
Rabbit
Reactivity
Human
Clonality
Monoclonal
Isotype
Rabbit IgG
Clone Number
024
Specificity
Human GOLM1
Purity/Purification
Protein A
Form/Format
Liquid; 0.2um filtered solution in PBS
Concentration
1mg/mL (varies by lot)
Applicable Applications for anti-GOLM1 antibody
ELISA (EIA), Flow Cytometry (FC)
Application Notes
FC: 1:25-1:100
ELISA: 1:5000-1:10,000
This antibody can be used at 1:5000-1:10000 with the appropriate secondary reagents to detect Human GOLM1.
Optimal concentrations/dilutions should be determined by the end user.
ELISA: 1:5000-1:10,000
This antibody can be used at 1:5000-1:10000 with the appropriate secondary reagents to detect Human GOLM1.
Optimal concentrations/dilutions should be determined by the end user.
Immunogen
Recombinant Human GOLM1/GP73 protein
Preparation
This antibody was obtained from a rabbit immunized with purified, recombinant Human GOLM1 (rh GOLM1).
Preparation and Storage
This antibody can be stored at 2-8°C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20°C to -80°C. Preservative-Free. Avoid repeated freeze-thaw cycles.
FCM (Flow Cytometry)
(Flow cytometric analysis of Human GOLM1(GP73) expression on HeLa cells. The cells were treated according to manufacturer's manual (BD Pharmingen'), stained with purified anti-Human GOLM1(GP73), then a FITC-conjugated second step antibody. The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of intact cells.)
FCM (Flow Cytometry)
(Flow cytometric analysis of Human GOLM1(GP73) expression on HeLa cells. The cells were treated according to manufacturer's manual (BD Pharmingen'), stained with purified anti-Human GOLM1(GP73), then a FITC-conjugated second step antibody. The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of intact cells.)
Related Product Information for anti-GOLM1 antibody
Golgi membrane protein 1, also known as Golgi membrane protein GP73, Golgi phosphoprotein 2, and GOLM1, is a protein that belongs to the GOLM1/CASC4 family. GOLM1 is widely expressed. It is highly expressed in the colon, prostate, trachea, and stomach. It is expressed at a lower level in testis, muscle, lymphoid tissues, white blood cells, and spleen. It is predominantly expressed by cells of the epithelial lineage. GOLM1 is expressed at a low level in the normal liver. Expression significantly increases in virus (HBV, HCV) infected liver. Expression of GOLM1 does not increase in liver disease due to non-viral causes (alcohol-induced liver disease, autoimmune hepatitis). Increased expression in hepatocytes appears to be a general feature of advanced liver disease. In liver tissue from patients with adult giant-cell hepatitis (GCH), GOLM1 is strongly expressed in hepatocyte-derived syncytial giant cells. GOLM1 is constitutively expressed by biliary epithelial cells but not by hepatocytes.
References
Kladney R.D., et al., (2000), GP73, a novel Golgi-localized protein upregulated by viral infection. Gene 249:53-65.Clark H.F., et al., (2003), The secreted protein discovery initiative (SPDI), a large-scale effort to identify novel human secreted and transmembrane proteins: a bioinformatics assessment.Genome Res. 13:2265-2270.Puri S., et al., (2002), Cycling of early Golgi proteins via the cell surface and endosomes upon lumenal pH disruption.Traffic 3:641-653.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Official Full Name
Golgi membrane protein 1
NCBI Official Synonym Full Names
golgi membrane protein 1
NCBI Official Symbol
GOLM1
NCBI Official Synonym Symbols
GP73; HEL46; GOLPH2; C9orf155; PSEC0257; bA379P1.3
NCBI Protein Information
Golgi membrane protein 1; golgi protein, 73-kD; golgi phosphoprotein 2; golgi membrane protein GP73; epididymis luminal protein 46
UniProt Protein Name
Golgi membrane protein 1
UniProt Gene Name
GOLM1
UniProt Synonym Gene Names
C9orf155; GOLPH2
UniProt Entry Name
GOLM1_HUMAN
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Product Notes
The GOLM1 golm1 (Catalog #AAA27750) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Recombinant Anti-GOLPH2/GOLM1 Antibody, Rabbit Monoclonal reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's GOLPH2/GOLM1 can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Flow Cytometry (FC). FC: 1:25-1:100 ELISA: 1:5000-1:10,000 This antibody can be used at 1:5000-1:10000 with the appropriate secondary reagents to detect Human GOLM1. Optimal concentrations/dilutions should be determined by the end user. Researchers should empirically determine the suitability of the GOLM1 golm1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "GOLPH2/GOLM1, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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