Mouse anti-Pig CSF1R Monoclonal Antibody | anti-CSF1R antibody

MOUSE ANTI PIG CSF1R: FITC

Cat. Num. AAA50257

• Gene Names: CSF1R; FMS; CSFR; FIM2; HDLS; C-FMS; CD115; CSF-1R; M-CSF-R
• Reactivity: Pig
• Applications: Flow Cytometry, Functional Assay
• Synonyms: CSF1R, Antibody; MOUSE ANTI PIG CSF1R: FITC; anti-CSF1R antibody

• Host: Mouse
• Reactivity: Pig
• Clonality: Monoclonal
• Isotype: IgG2a
• Clone Number: ROS8G11
• Specificity: CSF1R
• Form/Format: Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
• Concentration: IgG concentration 0.1 mg/ml (varies by lot)
• Sequence Length: 454

• Applicable Applications for anti-CSF1R antibody: FCM/FACS (Flow Cytometry)
• Reactivity Notes: Dog
• Preparation: Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
• Preservative Stabilisers: 0.09% Sodium Azide (NaN3), 1% Bovine Serum Albumin
• Buffer Solution: Phosphate buffered saline
• Preparation and Storage: Store at +4 degree C or at -20 degree C if preferred. Storage in frost-free freezers is not recommended. This product should be stored undiluted. This product is photosensitive and should be protected from light. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. ; Shelf Life: 18 months from date of despatch

FCM/FACS (Flow Cytometry)

(Staining of pig alveolar macrophages with Mouse anti Pig CSF1R followed by FITC conjugated Goat anti Mouse IgG following permeabilization with Leucoperm™ (BUF09))

FCM/FACS (Flow Cytometry)

(Staining of pig alveolar macrophages with Mouse anti Pig CSF1R)

FCM/FACS (Flow Cytometry)

(Staining of pig alveolar macrophages with Mouse anti Pig CSF1R)

FCM/FACS (Flow Cytometry)

(Staining of dog bone marrow derived macrophages (BMDMs) using Mouse anti pig CSF1-R, clone ROS 8G11 . Image courtesy of the Roslin Institute)

Related Product Information for anti-CSF1R antibody

Mouse anti Pig CSF1R, clone ROS8G11, recognizes the porcine homologue of human CSF1R, also known as CSF-1R, M-CSF receptor, CD115 and c-fms. CSF1R (CD115) is a single pass type 1 transmembrane protein belonging to the type III protein kinase family and is the cell surface receptor for both CSF1 and IL-34. Evidence from comparative sequence analysis across a range of vertebrate species indicates that CSF1 and IL-34 interact with distinct regions of CSF1R (Garceau et al. 2010). CSF1R cell surface expression appears to be one of the earliest events in macrophage lineage cell commitment and CSF1R expression appears primarily limited to macrophages (Garceau et al. 2010). The development of the monoclonal antibody ROS8G11 specifically recognizing porcine CSF1R provides an antibody that will assist with research into porcine monocyte and macrophage biology (Moffat et al. 2014). Clone ROS8G11 has been tested for cross reactivity in a limited number of species, and while it does not recognize human or equine CSFR1, further testing is required to conclude if the antibody recognizes CSF1R in other species such as canine.

NCBI and Uniprot Product Information

• NCBI GI #: 125858971
• NCBI GeneID: 1436
• Molecular Weight: 33,248 Da
• NCBI Official Full Name: CSF1R protein, partial
• NCBI Official Synonym Full Names: colony stimulating factor 1 receptor
• NCBI Official Symbol: CSF1R
• NCBI Official Synonym Symbols: FMS; CSFR; FIM2; HDLS; C-FMS; CD115; CSF-1R; M-CSF-R
• NCBI Protein Information: macrophage colony-stimulating factor 1 receptor
• UniProt Protein Name: Macrophage colony-stimulating factor 1 receptor
• UniProt Gene Name: CSF1R
• UniProt Synonym Gene Names: FMS; CSF-1-R; CSF-1R; M-CSF-R
• UniProt Entry Name: CSF1R_HUMAN