Application Data
(Chicken peripheral blood lymphocytes were stained with Mouse Anti-Chicken CD8α-FITC and Mouse Anti-Chicken CD4-PE .)
Mouse anti-Chicken CD8 alpha Monoclonal Antibody | anti-cd8a antibody
Mouse Anti-Chicken CD8a-FITC
Gene Names
cd8a; MGC136643; zgc:136643
Reactivity
Chicken
Applications
Immunoprecipitation, Immunohistochemistry, Flow Cytometry, Functional Assay
Synonyms
CD8 alpha, Antibody; Mouse Anti-Chicken CD8a-FITC; Mouse Anti-Chicken CD8a - FITC; Mouse Anti-Chicken CD8 alpha; anti-cd8a antibody
Host
Mouse
Reactivity
Chicken
Clonality
Monoclonal
Isotype
Mouse (BALB/c) IgG2b&kappa
Clone Number
EP72
Specificity
alpha chain of chicken CD8 (Mr 34 kDa)
In the chicken, the CD8 molecule is present in two forms: (i) a homodimer of two alpha chains, and (ii) a heterodimer of an alpha chain and a beta chain.1,2 Chicken CD8 is expressed on approximately 80% of thymocytes, 15% of blood mononuclear cells and 50% of spleen cells, but <1% of cells in the bursa and bone marrow.1 While the vast majority of CD8+ cells in the thymus, spleen, and blood of adult chickens express both CD8 alpha - and CD8 beta -chains, a relatively large proportion of the CD8+ TCR- gamma delta cells in the spleens of embryos and young chicks express only the alpha -chain of CD8. Among intestinal epithelial lymphocytes, the major CD8+ T cell populations present in mice are conserved, but there is a population of TCR- gamma delta CD8 alpha beta cells in the chicken that is not found in rodents.3 MAb EP72 recognizes the CD8 alpha chain.
In the chicken, the CD8 molecule is present in two forms: (i) a homodimer of two alpha chains, and (ii) a heterodimer of an alpha chain and a beta chain.1,2 Chicken CD8 is expressed on approximately 80% of thymocytes, 15% of blood mononuclear cells and 50% of spleen cells, but <1% of cells in the bursa and bone marrow.1 While the vast majority of CD8+ cells in the thymus, spleen, and blood of adult chickens express both CD8 alpha - and CD8 beta -chains, a relatively large proportion of the CD8+ TCR- gamma delta cells in the spleens of embryos and young chicks express only the alpha -chain of CD8. Among intestinal epithelial lymphocytes, the major CD8+ T cell populations present in mice are conserved, but there is a population of TCR- gamma delta CD8 alpha beta cells in the chicken that is not found in rodents.3 MAb EP72 recognizes the CD8 alpha chain.
Form/Format
Fluorescein (FITC) Conjugate
Sequence Length
216
Applicable Applications for anti-cd8a antibody
IP (Immunoprecipitation), IHC (Immunohistochemistry), FCM/FACS (Flow Cytometry)
Immunogen
Chicken splenocytes
Characterization
To ensure acceptable performance, each batch of monoclonal antibody is tested by flow cytometry to conform to characteristics of a standard reference reagent.
Preparation and Storage
The fluorescein (FITC) conjugate is supplied as 0.5 mg in 1.0 mL of PBS/NaN3. Store at 2-8 degree C.
Application Data
(Chicken peripheral blood lymphocytes were stained with Mouse Anti-Chicken CD8α-FITC and Mouse Anti-Chicken CD4-PE .)
Application Data
(Chicken peripheral blood lymphocytes were stained with Mouse Anti-Chicken CD8α-FITC and Mouse Anti-Chicken CD4-PE .)
Application Data
Application Data
References
1. Chan, M.L., C.-L. Chen, L. Ager, and M.D. Cooper. 1988. Identification of the avian homologues of mammalian CD4 and CD8 antigens. J. Immunol. 140:2133-2138.
2. Cooper, M.D., R.P. Bucy, and C.-L. Chen. 1990. In: The Avian Model in Developmental Biology: From Organism to Genes. Editions du CNRS, pp. 239-249.
3. Tregaskes, C.A., F. Kong, E. Paramithiotis, C.-L. Chen, M.J.H. Ratcliffe, T. F. Davison, and J.R. Young. 1995. Identification and analysis of the expression of CD8?? and CD8?? isoforms in chickens reveals a major TCR?? CD8?? subset of intestinal intraepithelial lymphocytes. J. Immunol. 154:4485-4494.
2. Cooper, M.D., R.P. Bucy, and C.-L. Chen. 1990. In: The Avian Model in Developmental Biology: From Organism to Genes. Editions du CNRS, pp. 239-249.
3. Tregaskes, C.A., F. Kong, E. Paramithiotis, C.-L. Chen, M.J.H. Ratcliffe, T. F. Davison, and J.R. Young. 1995. Identification and analysis of the expression of CD8?? and CD8?? isoforms in chickens reveals a major TCR?? CD8?? subset of intestinal intraepithelial lymphocytes. J. Immunol. 154:4485-4494.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
NCBI Official Full Name
CD8 antigen, alpha polypeptide
NCBI Official Synonym Full Names
CD8 antigen, alpha polypeptide
NCBI Official Symbol
cd8a
NCBI Official Synonym Symbols
MGC136643; zgc:136643
NCBI Protein Information
CD8 antigen, alpha polypeptide; CD8 alpha
UniProt Protein Name
CD8 antigen, alpha polypeptide
UniProt Gene Name
cd8a
UniProt Entry Name
Q24JW8_DANRE
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Product Notes
The cd8a cd8a (Catalog #AAA78708) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Mouse Anti-Chicken CD8a-FITC reacts with Chicken and may cross-react with other species as described in the data sheet. AAA Biotech's CD8 alpha can be used in a range of immunoassay formats including, but not limited to, IP (Immunoprecipitation), IHC (Immunohistochemistry), FCM/FACS (Flow Cytometry). Researchers should empirically determine the suitability of the cd8a cd8a for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CD8 alpha, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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