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product-image-AAA213502_IHC8.jpg IHC (Immunohistochemistry) (Detection of human CD68 by immunohistochemistry. Sample: FFPE section of human tonsil. Antibody: Mouse monoclonal anti-CD68 antibody [KP-1] (AAA213502) used at 1:100. Secondary: DyLight 594-conjugated goat anti-mouse IgG .)

CD68 recombinant antibody

Mouse anti-CD68 Recombinant Monoclonal Antibody, Purified [KP-1]

Gene Names
CD68; GP110; LAMP4; SCARD1
Reactivity
Human
Applications
Flow Cytometry, Functional Assay, Immunohistochemistry, Immunohistochemistry
Purity
Purified
Synonyms
CD68, Recombinant Antibody; Mouse anti-CD68 Recombinant Monoclonal Antibody, Purified [KP-1]; LAMP4; scavenger receptor class D, member 1; SCARD1; macrophage antigen CD68; GP110; CD68 antigen; CD antigen CD68; macrosialin; Macrosialin; CD68 molecule; CD68 recombinant antibody
Ordering
Host
Mouse
Reactivity
Human
Clonality
Monoclonal
Clone Number
KP-1
Purity/Purification
Purified
Form/Format
Liquid. Whole IgG. Phosphate Buffered Saline (PBS) with 0.1% BSA and 0.09% Sodium Azide
Concentration
50ug/ml (varies by lot)
Applicable Applications for CD68 recombinant antibody
FCM/FACS (Flow Cytometry), IHC (Immunohistochemistry), IHC (Immunohistochemistry)
Immunogen
Lysosomal fraction of human lung macrophages
Conjugation
Unconjugated
Preparation and Storage
Store at 2-8 degree C
Shelf Life: 1 year from date of receipt

IHC (Immunohistochemistry)

(Detection of human CD68 by immunohistochemistry. Sample: FFPE section of human tonsil. Antibody: Mouse monoclonal anti-CD68 antibody [KP-1] (AAA213502) used at 1:100. Secondary: DyLight 594-conjugated goat anti-mouse IgG .)

product-image-AAA213502_IHC8.jpg IHC (Immunohistochemistry) (Detection of human CD68 by immunohistochemistry. Sample: FFPE section of human tonsil. Antibody: Mouse monoclonal anti-CD68 antibody [KP-1] (AAA213502) used at 1:100. Secondary: DyLight 594-conjugated goat anti-mouse IgG .)

IHC (Immunohistochemistry)

(Detection of human CD68 (green) by immunohistochemistry. Sample: FFPE section of human colon carcinoma. Antibody: Mouse anti-CD68 monoclonal antibody [KP-1] (AAA213502) used at 1:500. Secondary: HRP-conjugated goat anti-mouse IgG . Substrate: Opal. Counterstain: DAPI (blue).)

product-image-AAA213502_IHC10.jpg IHC (Immunohistochemistry) (Detection of human CD68 (green) by immunohistochemistry. Sample: FFPE section of human colon carcinoma. Antibody: Mouse anti-CD68 monoclonal antibody [KP-1] (AAA213502) used at 1:500. Secondary: HRP-conjugated goat anti-mouse IgG . Substrate: Opal. Counterstain: DAPI (blue).)

IHC (Immunohistochemisry)

(Detection of human CD68 in FFPE tonsil by IHC. Antibody: Mouse monoclonal anti-CD68 [KP-1] (AAA213502). Secondary: HRP-conjugated goat anti-mouse IgG . Substrate: DAB.)

product-image-AAA213502_IHC11.jpg IHC (Immunohistochemisry) (Detection of human CD68 in FFPE tonsil by IHC. Antibody: Mouse monoclonal anti-CD68 [KP-1] (AAA213502). Secondary: HRP-conjugated goat anti-mouse IgG . Substrate: DAB.)

IHC (Immunohiostchemistry)

(Detection of human CD68 in FFPE seminoma by IHC. Antibody: Mouse monoclonal anti-CD68 [KP-1] (AAA213502). Secondary: HRP-conjugated goat anti-mouse IgG . Substrate: DAB. Counterstain:Hematoxylin (blue).)

product-image-AAA213502_IHC13.jpg IHC (Immunohiostchemistry) (Detection of human CD68 in FFPE seminoma by IHC. Antibody: Mouse monoclonal anti-CD68 [KP-1] (AAA213502). Secondary: HRP-conjugated goat anti-mouse IgG . Substrate: DAB. Counterstain:Hematoxylin (blue).)

FCM/FACS (Flow Cytometry)

(Detection of CD68 in human whole blood. Human whole blood was fixed in 4% formaldehyde. Red blood cells were lysed, and the sample was treated with 50% methanol. 1 X 10^6 cells were incubated with 2 ul of mouse monoclonal anti-CD68 antibody [KP-1] (AAA213502) (red), 0.1 ug mouse IgG1 isotype control (black), or no antibody (blue). Antibody binding was detected with 0.1 ug DyLight 488-conjugated goat anti-mouse IgG . The histogram represents gated granulocytes.)

product-image-AAA213502_FCM15.jpg FCM/FACS (Flow Cytometry) (Detection of CD68 in human whole blood. Human whole blood was fixed in 4% formaldehyde. Red blood cells were lysed, and the sample was treated with 50% methanol. 1 X 10^6 cells were incubated with 2 ul of mouse monoclonal anti-CD68 antibody [KP-1] (AAA213502) (red), 0.1 ug mouse IgG1 isotype control (black), or no antibody (blue). Antibody binding was detected with 0.1 ug DyLight 488-conjugated goat anti-mouse IgG . The histogram represents gated granulocytes.)
Related Product Information for CD68 recombinant antibody
CD68 is a 110-kD transmembrane glycoprotein that is highly expressed by human monocytes and tissue macrophages. It is a member of the lysosomal/endosomal-associated membrane glycoprotein (LAMP) family. The protein primarily localizes to lysosomes and endosomes with a smaller fraction circulating to the cell surface. It is a type I integral membrane protein with a heavily glycosylated extracellular domain and binds to tissue- and organ-specific lectins or selectins.CD68 is also a member of the scavenger receptor family. Scavenger receptors typically function to clear cellular debris, promote phagocytosis, and mediate the recruitment and activation of macrophages [taken from NCBI Entrez Gene (Gene ID: 968)].
Product Categories/Family for CD68 recombinant antibody

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
968
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
34,654 Da
NCBI Official Full Name
macrosialin isoform A
NCBI Official Synonym Full Names
CD68 molecule
NCBI Official Symbol
CD68
NCBI Official Synonym Symbols
GP110; LAMP4; SCARD1
NCBI Protein Information
macrosialin; CD68 antigen; macrophage antigen CD68; scavenger receptor class D, member 1
UniProt Protein Name
Macrosialin
UniProt Gene Name
CD68
UniProt Entry Name
CD68_HUMAN

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Product Notes

The CD68 cd68 (Catalog #AAA213502) is a Recombinant Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Mouse anti-CD68 Recombinant Monoclonal Antibody, Purified [KP-1] reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's CD68 can be used in a range of immunoassay formats including, but not limited to, FCM/FACS (Flow Cytometry), IHC (Immunohistochemistry), IHC (Immunohistochemistry). Researchers should empirically determine the suitability of the CD68 cd68 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CD68, Monoclonal Recombinant Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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