Application Data
(Staining of human peripheral blood lymphocytes with Mouse anti Human CD45:PECy5.5)
Mouse CD45 Monoclonal Antibody | anti-CD45 antibody
MOUSE ANTI HUMAN CD45:PerCP
Purified IgG conjugated to Peridinin Chlorophyll protein (PerCP) - lyophilised
Perservative Stabilisers
Fusion Partners
Shelf Life: 12 months from date of reconstitution.
Application Data
(Staining of human peripheral blood lymphocytes with Mouse anti Human CD45:PECy5.5)
Application Data
(Staining of human peripheral blood lymphocytes with Mouse anti Human CD45:PECy5.5)
Application Data
(Staining of human peripheral blood lymphocytes with Mouse anti Human CD45:RPE)
Application Data
(Staining of human peripheral blood lymphocytes with Mouse anti Human CD45:RPE)
Application Data
(Staining of human peripheral blood lymphocytes with Mouse anti Human CD45:FITC)
Application Data
(Staining of human peripheral blood lymphocytes with Mouse anti Human CD45:FITC)
Application Data
(Staining of human peripheral blood lymphocytes with Mouse anti Human CD45:Biotin)
Application Data
(Staining of human peripheral blood lymphocytes with Mouse anti Human CD45:Biotin)
Application Data
(Staining of human peripheral blood lymphocytes with Mouse anti Human CD45:APC)
Application Data
(Staining of human peripheral blood lymphocytes with Mouse anti Human CD45:APC)
Application Data
(Staining of human peripheral blood lymphocytes with Mouse anti HumanCD45:Alexa FluorR 488)
Application Data
(Staining of human peripheral blood lymphocytes with Mouse anti HumanCD45:Alexa FluorR 488)
Application Data
(Staining of human peripheral blood granulocytes with Mouse anti Human CD45:Alexa FluorR 647)
Application Data
(Staining of human peripheral blood granulocytes with Mouse anti Human CD45:Alexa FluorR 647)
Application Data
(Staining of human peripheral blood granulocytes with Mouse anti Human CD45: Pacific BlueR)
Application Data
(Staining of human peripheral blood granulocytes with Mouse anti Human CD45: Pacific BlueR)
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Product Notes
The CD45 ptprc (Catalog #AAA12246) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's CD45 can be used in a range of immunoassay formats including, but not limited to, FCM/FACS (Flow Cytometry). Flow Cytometry: Use 10ul of the suggested working dilution to label 106 cells or 100ul whole blood. Researchers should empirically determine the suitability of the CD45 ptprc for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CD45, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Frequently Asked Questions
What cell types express CD45 and can be detected with this antibody?
CD45 is a pan-leukocyte marker expressed on all nucleated white blood cells (lymphocytes, neutrophils, monocytes, macrophages, dendritic cells, eosinophils, basophils). It displays variable intensity: highest on lymphocytes, intermediate on monocytes, lower on neutrophils, and blast cells. CD45 is absent on erythrocytes, platelets, and non-hematopoietic cells. AAA Biotech's CD45 antibody detects all leukocyte lineages across these expression levels.
Can CD45 antibody be used to distinguish leukocytes from other cells in flow cytometry?
Yes, CD45 is the primary marker for leukocyte identification in flow cytometry. CD45/side-scatter (SSC) gating efficiently identifies blast cells and distinguishes hematopoietic from non-hematopoietic populations. CD45 expression intensity combined with forward scatter (FSC) separates lymphocytes (high CD45, low FSC), monocytes (intermediate), and neutrophils (variable CD45, high SSC). This enables rapid immunophenotyping and disease diagnosis in bone marrow and blood samples.
Is this CD45 monoclonal antibody suitable for immunohistochemistry or immunofluorescence?
Yes, CD45 antibodies perform well in immunohistochemistry (IHC) and immunofluorescence (IF) on paraffin-embedded and frozen tissues. CD45 marks inflammatory cell infiltration in kidneys, livers, lungs, and tumor microenvironments. Standard antigen retrieval protocols enhance signal in formalin-fixed tissue. Recommend 1:50-1:200 dilutions for IHC. CD45 IF requires permeabilization to access cytoplasmic epitopes; co-staining validates leukocyte identification.
How does CD45 expression change during immune activation or disease?
CD45 expression generally remains stable as a surface marker, but its functional state alters during activation. CD45 dephosphorylates inhibitory kinases, enabling T-cell receptor signaling during immune activation. In diabetes, infection, or inflammatory diseases, the CD45+ infiltrate dramatically increases in affected tissues. CD45 levels correlate with disease severity; elevated infiltration indicates immune dysregulation. Quantifying CD45+ cells monitors therapeutic efficacy and disease progression.
Can CD45 antibody differentiate between lymphocyte subpopulations?
CD45 alone cannot differentiate lymphocyte subsets; it identifies all leukocytes without subset specificity. However, CD45-based gating enables subsequent analysis with lineage-specific markers (CD3 for T-cells, CD19 for B-cells, CD56 for NK cells). AAA Biotech recommends combining CD45 with subset-specific antibodies in multiparameter flow cytometry panels to identify CD45+CD3+, CD45+CD19+, and CD45+CD56+ populations precisely.
What dilution or concentration is recommended for best results in flow cytometry?
Optimal dilutions vary by clone, host species, and conjugate. Typical recommendations: 5-10 μL per 10⁶ cells for directly conjugated antibodies, or 1:100-1:500 for unconjugated primary antibodies. Titrate antibody to determine optimal dilution maximizing signal-to-noise ratio. Use fluorescence minus one (FMO) controls to assess background. AAA Biotech datasheets provide specific dilution ranges; validate experimentally for your instrument and staining protocol.
What cell types express CD45 and can be detected with a mouse CD45 antibody?
CD45, also known as the Leukocyte Common Antigen (LCA), is expressed on virtually all hematopoietic cells except for mature erythrocytes and platelets. This includes B cells, T cells, monocytes, granulocytes, and NK cells. The AAA Biotech mouse CD45 antibody is a standard tool for identifying these immune populations in various mouse tissue samples or cell cultures.
Can a CD45 monoclonal antibody be used for flow cytometry analysis?
Yes, CD45 monoclonal antibodies are a cornerstone of flow cytometry. They are frequently used as a primary gating marker to distinguish leukocytes from non-hematopoietic cells, such as epithelial or tumor cells. AAA Biotech provides CD45 antibodies that are optimized for flow cytometry, allowing for the clear separation and identification of the total white blood cell population.
Is CD45 a universal marker for all leukocytes?
Yes, CD45 is considered a universal pan-leukocyte marker. Its presence is mandatory for the activation of T and B cell receptors. Because it is expressed across the entire leukocyte lineage, researchers using AAA Biotech products rely on CD45 to confirm the purity of immune cell isolations or to quantify the infiltration of immune cells into tissues.
Can CD45 antibody distinguish between different immune cell populations?
While CD45 identifies the total leukocyte population, it cannot distinguish between specific subsets like T cells and B cells on its own. To differentiate these, CD45 must be used in a multi-color panel alongside other markers such as CD3, CD4, or CD19. AAA Biotech antibodies are designed to work effectively in these multi-marker panels for detailed immune profiling.
Is mouse CD45 antibody suitable for immunohistochemistry or immunofluorescence?
Yes, mouse CD45 antibodies are validated for IHC and IF. In IHC, they are used to stain immune cells within tissue sections, such as the spleen, lymph nodes, or inflamed organs. AAA Biotech’s antibody allows for the clear visualization of leukocyte localization and density within the tissue architecture, which is vital for studying inflammation and immune responses.
How does CD45 expression change in inflammation or immune activation?
While CD45 is constitutively expressed, its isoforms and surface density can change during the activation of immune cells. For example, specific isoforms like CD45RO or CD45RA are associated with memory and naive T cells, respectively. Using AAA Biotech antibodies to monitor these changes helps researchers understand the functional state of the immune system during infection or chronic inflammatory disease.
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