IF (Immunofluorescence)
(Immunofluorescence of HaCaT cells stained with Hoechst 3342 (Blue) for nucleus staining and monoclonal anti-human beta-Tubulin antibody (1:1000) with Texas Red (Red). Immunofluorescence of A431 cells stained with DAPI (Blue) for nucleus staining and monoclonal anti-human beta-Tubulin antibody (1:500) with Alexa Fluor 488 (Green). Immunofluorescence of Balb/3T3 cells stained with DAPI (Blue) for nucleus staining and monoclonal anti-human beta-Tubulin antibody (1:200) with Alexa Fluor 488 (Green).)
Mouse anti-Human, Mouse beta-Tubulin Monoclonal Antibody | anti-TUBB2B antibody
beta-Tubulin antibody
Gene Names
TUBB2B; CDCBM7; PMGYSA; bA506K6.1
Reactivity
Human, Mouse
Applications
Immunofluorescence, Western Blot, ELISA
Purity
By protein-G affinity chromatography
Synonyms
beta-Tubulin, Antibody; beta-Tubulin antibody; DKFZp566F223; FLJ98847; MGC8685; Tubulin beta-2B chain; bA506K6.1; TUBB2B; Tubulin beta 2b; Tubulin beta-2B; anti-TUBB2B antibody
Host
Mouse
Reactivity
Human, Mouse
Clonality
Monoclonal
Isotype
IgG2b,k
Clone Number
AT5B2
Purity/Purification
By protein-G affinity chromatography
Form/Format
Liquid. In phosphate-buffered saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol.
Concentration
1mg/ml (varies by lot)
Sequence Length
445
Applicable Applications for anti-TUBB2B antibody
IF (Immunofluorescence), WB (Western Blot), ELISA
Antigen Species
Human
Immunogen
Recombinant human beta-Tubulin (1-445aa) purified from E. coli
Clone Note
Anti-human beta-Tubulin mAb, clone AT5B2, is derived from hybridization of mouse F0 myeloma cells with spleen cells from BALB/c mice immunized with a recombinant human beta-Tubulin protein.
Preparation and Storage
Can be stored at 4 degree C.
For long term storage, aliquot and store at -20 degree C.
Avoid repeated freezing and thawing cycles.
For long term storage, aliquot and store at -20 degree C.
Avoid repeated freezing and thawing cycles.
IF (Immunofluorescence)
(Immunofluorescence of HaCaT cells stained with Hoechst 3342 (Blue) for nucleus staining and monoclonal anti-human beta-Tubulin antibody (1:1000) with Texas Red (Red). Immunofluorescence of A431 cells stained with DAPI (Blue) for nucleus staining and monoclonal anti-human beta-Tubulin antibody (1:500) with Alexa Fluor 488 (Green). Immunofluorescence of Balb/3T3 cells stained with DAPI (Blue) for nucleus staining and monoclonal anti-human beta-Tubulin antibody (1:200) with Alexa Fluor 488 (Green).)
IF (Immunofluorescence)
(Immunofluorescence of HaCaT cells stained with Hoechst 3342 (Blue) for nucleus staining and monoclonal anti-human beta-Tubulin antibody (1:1000) with Texas Red (Red). Immunofluorescence of A431 cells stained with DAPI (Blue) for nucleus staining and monoclonal anti-human beta-Tubulin antibody (1:500) with Alexa Fluor 488 (Green). Immunofluorescence of Balb/3T3 cells stained with DAPI (Blue) for nucleus staining and monoclonal anti-human beta-Tubulin antibody (1:200) with Alexa Fluor 488 (Green).)
WB (Western Blot)
(The Cell lysate (40ug) and Mouse tissue extracts (35ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human beta-Tubulin (1:5000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1.: Jurkat cell lysate Lane 2.: A549 cell lysate Lane 3.: A431 cell lysate Lane 4.: HepG2 cell lysate Lane 5.: Mouse Brain tissue extract)
WB (Western Blot)
(The Cell lysate (40ug) and Mouse tissue extracts (35ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human beta-Tubulin (1:5000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1.: Jurkat cell lysate Lane 2.: A549 cell lysate Lane 3.: A431 cell lysate Lane 4.: HepG2 cell lysate Lane 5.: Mouse Brain tissue extract)
Related Product Information for anti-TUBB2B antibody
Tubulin is a major cytoskeleton component that has five distinct forms. Multiple beta Tubulin isoforms have been characterized and are expressed in mammalian tissues. Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha-chain.
Product Categories/Family for anti-TUBB2B antibody
References
Xavier Hubert Jaglin, et al. (2009) Nat Genet, 41 (6):746-52.; Bouwmeester T, et al. (2004) Nat Cell Bio, 6 (2):97-105.; Modig, C, et al. (1999) Cell Motil Cytoskeleton, 42 (4): 315-330.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
49,953 Da
NCBI Official Full Name
tubulin beta-2B chain
NCBI Official Synonym Full Names
tubulin beta 2B class IIb
NCBI Official Symbol
TUBB2B
NCBI Official Synonym Symbols
CDCBM7; PMGYSA; bA506K6.1
NCBI Protein Information
tubulin beta-2B chain
UniProt Protein Name
Tubulin beta-2B chain
UniProt Gene Name
TUBB2B
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Product Notes
The TUBB2B tubb2b (Catalog #AAA48084) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The beta-Tubulin antibody reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's beta-Tubulin can be used in a range of immunoassay formats including, but not limited to, IF (Immunofluorescence), WB (Western Blot), ELISA. Researchers should empirically determine the suitability of the TUBB2B tubb2b for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "beta-Tubulin, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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