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ICC (Immunocytochemistry)
(ICC staining Phospho-AMPK alpha 2 (S345) in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
Rabbit AMPK alpha 2 Monoclonal Antibody | anti-PRKAA2 antibody
AMPK alpha 2 (Phospho-S345) Antibody
Gene Names
PRKAA2; AMPK; AMPK2; PRKAA; AMPKa2
Reactivity
Human, Mouse, Rat
Applications
Immunohistochemistry, Immunofluorescence, Immunocytochemistry, Immunohistochemistry, Western Blot
Purity
ProA affinity purified
Synonyms
AMPK alpha 2, Antibody; AMPK alpha 2 (Phospho-S345) Antibody; 5'-AMP-activated protein kinase catalytic subunit alpha-2 antibody; AAPK2_HUMAN antibody; ACACA kinase antibody; Acetyl-CoA carboxylase kinase antibody; AMPK alpha 2 chain antibody; AMPK subunit alpha-2 antibody; AMPK2 antibody; AMPKa2 antibody; AMPKalpha2 antibody; HMGCR kinase antibody; Hydroxymethylglutaryl-CoA reductase kinase antibody; PRKAA antibody; PRKAA2 antibody; Protein kinase AMP activated alpha 2 catalytic subunit antibody; Protein kinase AMP activated catalytic subunit alpha 2 antibody; anti-PRKAA2 antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Monoclonal
Clone Number
JJ08-19
Purity/Purification
ProA affinity purified
Form/Format
1*TBS (pH7.4), 1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Applicable Applications for anti-PRKAA2 antibody
IHC (Immunohistochemistry), IF (Immunofluorescence), ICC (Immunocytochemistry), IHC (Immunohistochemistry), WB (Western Blot)
Antibody Type
Recombinant Antibody
Immunogen
Synthetic phospho-peptide corresponding to residues surrounding Ser345 of human AMPK alpha 2.
Conjugation
Unconjugated
Preparation and Storage
Store at -20 degree C.
ICC (Immunocytochemistry)
(ICC staining Phospho-AMPK alpha 2 (S345) in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
ICC (Immunocytochemistry)
(ICC staining Phospho-AMPK alpha 2 (S345) in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
ICC (Immunocytochemistry)
(ICC staining Phospho-AMPK alpha 2 (S345) in 293 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
ICC (Immunocytochemistry)
(ICC staining Phospho-AMPK alpha 2 (S345) in 293 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
IHC (Immunohiostchemistry)
(Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue using anti-Phospho-AMPK alpha 2 (S345) antibody. Counter stained with hematoxylin.)
IHC (Immunohiostchemistry)
(Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue using anti-Phospho-AMPK alpha 2 (S345) antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry)
(Immunohistochemical analysis of paraffin-embedded human pancreas tissue using anti-Phospho-AMPK alpha 2 (S345) antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry)
(Immunohistochemical analysis of paraffin-embedded human pancreas tissue using anti-Phospho-AMPK alpha 2 (S345) antibody. Counter stained with hematoxylin.)
Related Product Information for anti-PRKAA2 antibody
Five-prime-AMP-activated protein kinase, known as AMPK, is a heterotrimeric complex that comprises of a catalytic alpha subunit, and regulatory beta and gamma. AMPK protects cells from stresses that cause ATP depletion by switching off ATP-consuming biosynthetic pathways. AMPK is activated by high AMP and low ATP via a mechanism involving allosteric regulation, promotion of phosphorylation by an upstream protein kinase known as AMPK kinase (AMPKK), and inhibition of dephosphorylation. Activated AMPK can phosphorylate and regulate in vivo hydroxymethylglutaryl-CoA reductase and acetyl-CoA carboxylase, which are key regulatory enzymes of sterol synthesis and fatty acid synthesis, respectively. The human AMPKalpha1 gene maps to chromosome 5p12 and encodes a 548 amino acid protein. The major regulatory site phosphorylated by AMPKK on AMPKalpha has been identified as Thr 172 within the activation loop between the DFG and APE motifs of the alpha-subunits.
Product Categories/Family for anti-PRKAA2 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
62,320 Da
NCBI Official Full Name
AMP-activated protein kinase, AMPK
NCBI Official Synonym Full Names
protein kinase, AMP-activated, alpha 2 catalytic subunit
NCBI Official Symbol
PRKAA2
NCBI Official Synonym Symbols
AMPK; AMPK2; PRKAA; AMPKa2
NCBI Protein Information
5'-AMP-activated protein kinase catalytic subunit alpha-2; ACACA kinase; HMGCR kinase; AMPK-alpha-2 chain; AMPK subunit alpha-2; acetyl-CoA carboxylase kinase; hydroxymethylglutaryl-CoA reductase kinase; AMP-activated protein kinase alpha-2 subunit varian
UniProt Protein Name
5'-AMP-activated protein kinase catalytic subunit alpha-2
UniProt Gene Name
PRKAA2
UniProt Synonym Gene Names
AMPK; AMPK2; AMPK subunit alpha-2; ACACA kinase; HMGCR kinase
UniProt Entry Name
AAPK2_HUMAN
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Product Notes
The PRKAA2 prkaa2 (Catalog #AAA311042) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The AMPK alpha 2 (Phospho-S345) Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's AMPK alpha 2 can be used in a range of immunoassay formats including, but not limited to, IHC (Immunohistochemistry), IF (Immunofluorescence), ICC (Immunocytochemistry), IHC (Immunohistochemistry), WB (Western Blot). Researchers should empirically determine the suitability of the PRKAA2 prkaa2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "AMPK alpha 2, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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