Standard Curve (Sample)
Mouse IL-12(p40) ELISA Kit | IL12B elisa kit
Mouse IL-12(p40) PicoKine ELISA Kit
Gene Names
Il12b; p40; Il-12b; Il12p40; Il-12p40
Reactivity
Mouse
Synonyms
IL-12(p40); N/A; Mouse IL-12(p40) PicoKine ELISA Kit; Interleukin-12 subunit beta; interleukin 12B; 40 kD Subunit; CLMF; CLMF 2; CLMF p40; CLMF2; Cytotoxic lymphocyte maturation factor 2; Cytotoxic lymphocyte maturation factor 2 p40; Cytotoxic lymphocyte maturation factor 40 kDa subunit; IL 12 subunit p40; IL 12B; IL-12 subunit p40; IL-12B; IL12 subunit p40; IL12B; IL12B_HUMAN; Interleukin 12 beta chain; Interleukin 12 p40; Interleukin 12 subunit beta; Interleukin 12B; Interleukin12B; Natural killer cell stimulatory factor 2; Natural killer cell stimulatory factor 40 kD subunit; NK cell stimulatory factor chain 2; NKSF; NKSF 2; NKSF2; p40; Subunit P40; T cell stimulating factor; TcMF; TSF; IL12B elisa kit
Reactivity
Mouse
Specificity
Natural and recombinant Mouse Il12b
Sequence Length
335
Samples
Cell culture supernatants, serum and plasma (heparin, EDTA, citrate)
Detection Range
15.6 pg/ml - 1,000 pg/ml
Sensitivity
<10 pg/ml
Intra-assay Precision
Three samples of known concentration were tested on one plate to assess intra-assay precision.
Inter-assay Precision
Three samples of known concentration were tested in separate assays to assess inter-assay precision.
Preparation and Storage
Store at 4 degree C for 6 months, at -20 degree C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
Standard Curve (Sample)
Standard Curve (Sample)
Related Product Information for IL12B elisa kit
Background: Interleukin-12 (IL12, formerly NKSF, for natural killer cell stimulatory factor, or CLMF, for cytotoxic lymphocyte maturation factor) is a novel cytokine cloned from B-cell lines. IL-12 is a heterodimeric molecule composed of p35 and p40 subunits.1 The larger 40-kDa subunit (p40) is a member of the cytokine receptor family, and the smaller 35-kDa subunit (p35) is related to IL6 and GCSF.2 Both IL-12 p40 (-/-) and p35 (-/-) mice fail to produce IL-12 p70 heterodimer.3 Interleukin (IL)-12 has been cloned on the basis of its ability to activate natural killer (NK) cells and promote the development of cytolytic T cells. With further understanding of its activities, IL-12 has emerged as an important cytokine, affecting both immune and hematologic functions. It has been shown to be necessary for the T cell independent induction of interferon (IFN)-gamma, critical for the initial suppression of bacterial and parasitic infection; for the development of a Th1 response, critical for effective host defense against intracellular pathogens; and for the activation of differentiated T lymphocytes of both CD4+ and CD8+ phenotype.
Principle of the Assay: The Picokine™ Mouse Il12b Pre-Coated ELISA (Enzyme-Linked Immunosorbent Assay) kit is a solid-phase immunoassay specially designed to measure Mouse Il12b with a 96-well strip plate that is pre-coated with antibody specific for Il12b. The detection antibody is a biotinylated antibody specific for Il12b. The capture antibody is monoclonal antibody from rat and the detection antibody is polyclonal antibody from goat. The kit contains recombinant Mouse Il12b with immunogen: Expression system for standard: sf21; Immunogen sequence: M23-S335. The kit is analytically validated with ready-to-use reagents. To measure Mouse Il12b, add standards and samples to the wells, then add the biotinylated detection antibody. Wash the wells with PBS or TBS buffer, and add Avidin-Biotin-Peroxidase Complex (ABC-HRP). Wash away the unbounded ABC-HRP with PBS or TBS buffer and add TMB. TMB is an HRP substrate and will be catalyzed to produce a blue color product, which changes into yellow after adding the acidic stop solution. The absorbance of the yellow product at 450nm is linearly proportional to Mouse Il12b in the sample. Read the absorbance of the yellow product in each well using a plate reader, and benchmark the sample wells' readings against the standard curve to determine the concentration of Mouse Il12b in the sample.
Principle of the Assay: The Picokine™ Mouse Il12b Pre-Coated ELISA (Enzyme-Linked Immunosorbent Assay) kit is a solid-phase immunoassay specially designed to measure Mouse Il12b with a 96-well strip plate that is pre-coated with antibody specific for Il12b. The detection antibody is a biotinylated antibody specific for Il12b. The capture antibody is monoclonal antibody from rat and the detection antibody is polyclonal antibody from goat. The kit contains recombinant Mouse Il12b with immunogen: Expression system for standard: sf21; Immunogen sequence: M23-S335. The kit is analytically validated with ready-to-use reagents. To measure Mouse Il12b, add standards and samples to the wells, then add the biotinylated detection antibody. Wash the wells with PBS or TBS buffer, and add Avidin-Biotin-Peroxidase Complex (ABC-HRP). Wash away the unbounded ABC-HRP with PBS or TBS buffer and add TMB. TMB is an HRP substrate and will be catalyzed to produce a blue color product, which changes into yellow after adding the acidic stop solution. The absorbance of the yellow product at 450nm is linearly proportional to Mouse Il12b in the sample. Read the absorbance of the yellow product in each well using a plate reader, and benchmark the sample wells' readings against the standard curve to determine the concentration of Mouse Il12b in the sample.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
38,235 Da
NCBI Official Full Name
Interleukin-12 subunit beta
NCBI Official Synonym Full Names
interleukin 12b
NCBI Official Symbol
Il12b
NCBI Official Synonym Symbols
p40; Il-12b; Il12p40; Il-12p40
NCBI Protein Information
interleukin-12 subunit beta; CLMF p40; IL-12 p40; IL-12 subunit p40; IL-23 subunit p40; cytotoxic lymphocyte maturation factor 40 kDa subunit
UniProt Protein Name
Interleukin-12 subunit beta
UniProt Gene Name
Il12b
UniProt Synonym Gene Names
IL-12B; CLMF p40
UniProt Entry Name
IL12B_MOUSE
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Product Notes
The Mouse IL12B il12b (Catalog #AAA45587) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA45587 ELISA Kit recognizes Mouse IL12B. It is sometimes possible for the material contained within the vial of "IL-12(p40), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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