Standard Curve (Sample)
(Mouse IL-12(p70) PicoKine ELISA Kit standard curve)
Mouse IL-12(p70) ELISA Kit | IL12A elisa kit
Mouse IL-12(p70) PicoKine ELISA Kit
Gene Names
Il12a; p35; Ll12a; Il-12a; IL-12p35
Reactivity
Mouse
Synonyms
IL-12(p70); N/A; Mouse IL-12(p70) PicoKine ELISA Kit; Interleukin-12 subunit alpha; interleukin 12A; CLMF; CLMF p35; Cytotoxic lymphocyte maturation factor 1; Cytotoxic lymphocyte maturation factor 1 p35; Cytotoxic lymphocyte maturation factor 35 kDa subunit; IL-12 subunit p35; IL-12A; IL12A; IL12A_HUMAN; IL35 subunit; Interleukin 12 alpha chain; Interleukin 12 p35; Interleukin 12 p70; Natural killer cell stimulatory factor 1; Natural killer cell stimulatory factor 1 35 kD subunit; NF cell stimulatory factor chain 1; NFSK; NK cell stimulatory factor chain 1; NKSF1; P35; IL12A elisa kit
Reactivity
Mouse
Specificity
Natural and recombinant mouse IL-12(P70)
Sequence Length
236
Samples
Cell culture supernates and serum
Assay Type
Sandwich
Detection Range
15.6pg/ml-1000pg/ml
Sensitivity
< 1.5pg/ml
Preparation and Storage
Store at 4 degree C for 6 months, at -20 degree C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
Standard Curve (Sample)
(Mouse IL-12(p70) PicoKine ELISA Kit standard curve)
Standard Curve (Sample)
(Mouse IL-12(p70) PicoKine ELISA Kit standard curve)
Related Product Information for IL12A elisa kit
Principle of the assay: mouse IL-12(P70) ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from rat specific for IL-12(P70) has been precoated onto 96-well plates. Standards (sf21,(M23-S335)+(R23-A215)) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for IL-12(P70) is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the mouse IL-12(P70) amount of sample captured in plate.
Background: Interleukin (IL)-12 is a 70-KDa cytokine comprised of two disulfide-linked proteins (p35 and p40) and is essential for the initiation of effective immune response. And the IL-12p70 is a heterodimer of p35 and p40 subunits; it is an important cytokine secreted by antigen-presenting cells in response to antigenic stimulation. Gene expression analysis of the IL-12 cytokine family subunits revealed that both strains induced high levels of p40 (protein chain communal to IL-12 p70 and IL-23) as well as p19, a subunit of IL-23. Conversely only ACT- 18HS19 infection induced consistent transcription of IL-12 p35, a subunit of IL-12 p70. The standard product used in this kit is recombinant mouse IL-12 p70 comprising of a 193 amino acids p35 chain and a 313 amino acids p40 chain connected by a disulfide bond.
Background: Interleukin (IL)-12 is a 70-KDa cytokine comprised of two disulfide-linked proteins (p35 and p40) and is essential for the initiation of effective immune response. And the IL-12p70 is a heterodimer of p35 and p40 subunits; it is an important cytokine secreted by antigen-presenting cells in response to antigenic stimulation. Gene expression analysis of the IL-12 cytokine family subunits revealed that both strains induced high levels of p40 (protein chain communal to IL-12 p70 and IL-23) as well as p19, a subunit of IL-23. Conversely only ACT- 18HS19 infection induced consistent transcription of IL-12 p35, a subunit of IL-12 p70. The standard product used in this kit is recombinant mouse IL-12 p70 comprising of a 193 amino acids p35 chain and a 313 amino acids p40 chain connected by a disulfide bond.
NCBI and Uniprot Product Information
NCBI GeneID
Molecular Weight
26,204 Da
NCBI Official Synonym Full Names
interleukin 12a
NCBI Official Symbol
Il12a
NCBI Official Synonym Symbols
p35; Ll12a; Il-12a; IL-12p35
NCBI Protein Information
interleukin-12 subunit alpha; CLMF p35; IL-12 subunit p35; interleukin 12a p35 subunit; cytotoxic lymphocyte maturation factor 35 kDa subunit
UniProt Protein Name
Interleukin-12 subunit alpha
UniProt Gene Name
Il12a
UniProt Entry Name
F8WI71_MOUSE
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Product Notes
The Mouse IL12A il12a (Catalog #AAA45242) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA45242 ELISA Kit recognizes Mouse IL12A. It is sometimes possible for the material contained within the vial of "IL-12(p70), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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