Standard Curve (Sample)
Beta Actin (ACTB) CLIA Kit | ACTB clia kit
Multi-species Beta Actin (ACTB) CLIA Kit
Reactivity
Human, Mouse, Rat
Synonyms
Beta Actin (ACTB); N/A; Multi-species Beta Actin (ACTB) CLIA Kit; Human/Mouse/Rat Beta Actin (ACTB) (Sandwich CLIA) CLIA Kit; Actin Beta; ACT-B; PS1TP5BP1; Actin, cytoplasmic 1; Actin, cytoplasmic 1, N-terminally processed; ACTB clia kit
Reactivity
Human, Mouse, Rat
Specificity
This assay has high sensitivity and excellent specificity for detection of Actin Beta (ACTb).
No significant cross-reactivity or interference between Actin Beta (ACTb) and analogues was observed.
No significant cross-reactivity or interference between Actin Beta (ACTb) and analogues was observed.
Assay Type
Double-antibody Sandwich
Samples
Tissue homogenates, Cell lysates, Cell culture supernates and other biological fluids
Detection Range
0.14-100ng/mL
Sensitivity
0.06ng/mL
Application
Chemiluminescent immunoassay for Antigen Detection.
Intra-assay Precision (Precision within an assay)
3 samples with low, middle and high level Actin Beta (ACTb) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays)
3 samples with low, middle and high level Actin Beta (ACTb) were tested on 3 different plates, 8 replicates in each plate.
CV(%) =
SD/meanX100
Intra-Assay
CV<10%
Inter-Assay
CV<12%
Preparation and Storage
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Standard Curve (Sample)
Standard Curve (Sample)
Related Product Information for ACTB clia kit
The microplate provided in this kit has been pre-coated with an antibody specific to Actin Beta (ACTb). Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to Actin Beta (ACTb). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the Actin Beta (ACTb) level in the sample or standard.;
Product Categories/Family for ACTB clia kit
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Product Notes
The Human, Mouse, Rat ACTB (Catalog #AAA140952) is a CLIA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA140952 CLIA Kit recognizes Human, Mouse, Rat ACTB. It is sometimes possible for the material contained within the vial of "Beta Actin (ACTB), CLIA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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