Human S100 Calcium Binding Protein B (S100B) ELISA Kit | S100B elisa kit

Human S100 Calcium Binding Protein B (S100B) Mini Samples ELISA Kit

Cat. Num. AAA190259

• Gene Names: S100B; NEF; S100; S100-B; S100beta
• Reactivity: Human
• Synonyms: S100 Calcium Binding Protein B (S100B); N/A; Human S100 Calcium Binding Protein B (S100B) Mini Samples ELISA Kit; S100B Mini-sample ELISA kit (Human); S100 Calcium Binding Protein B; S100-B; NEF; S100Beta; S-100 protein subunit beta; S100B elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of Mini Samples S100 Calcium Binding Protein B (S100B). No significant cross-reactivity or interference between Mini Samples S100 Calcium Binding Protein B (S100B) and analogues was observed.; Reactive with Human S100B/S100 Calcium Binding Protein B
• Assay Type: Double-antibody Sandwich
• Samples: Serum, Plasma, Tissue homogenates, Cell lysates, Cell culture supernates and other biological fluids
• Detection Range: 0.156-10ng/mL
• Sensitivity: 0.054ng/mL
• Sample Size: 15-25uL
• Detection Method: Colormetric
• Featured Series: ME kit

• Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Mini Samples S100 Calcium Binding Protein B (S100B) were tested 20 times on one plate, respectively.; Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Mini Samples S100 Calcium Binding Protein B (S100B) were tested on 3 different plates, 8 replicates in each plate.; CV(%) = SD/meanX100; Intra-Assay: CV<10%, Inter-Assay: CV<12%
• Function: Detects protein (micro-volume samples 15-25 ul)
• Assay Procedure Summary: 1. Prepare all reagents, samples and standards; 2. Add 25ul standard or sample to each well. Incubate 1 hour at 37 degree C; 3. Aspirate and add 25ul prepared Detection Reagent A. Incubate 1 hour at 37 degree C; 4. Aspirate and wash 3 times; 5. Add 25ul prepared Detection Reagent B. Incubate 30 minutes at 37 degree C; 6. Aspirate and wash 5 times; 7. Add 25ul Substrate Solution. Incubate 10-20 minutes at 37 degree C; 8. Add 20ul Stop Solution. Read at 450nm immediately.
• Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mini Samples S100 Calcium Binding Protein B (S100B). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Mini Samples S100 Calcium Binding Protein B (S100B). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mini Samples S100 Calcium Binding Protein B (S100B), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Mini Samples S100 Calcium Binding Protein B (S100B) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• Research Area: Tumor immunity, Infection immunity, Neuro science
• Preparation and Storage: Store at 4 degree C for 1 month/-20 degree C for long-term (One year within shelf life); The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Standard Curve (Sample)

NCBI and Uniprot Product Information

• NCBI GI #: 12804681
• NCBI GeneID: 6285
• UniProt Accession #: P04271; D3DSN6
• Molecular Weight: 10,713 Da
• NCBI Official Full Name: S100 calcium binding protein B
• NCBI Official Synonym Full Names: S100 calcium binding protein B
• NCBI Official Symbol: S100B
• NCBI Official Synonym Symbols: NEF; S100; S100-B; S100beta
• NCBI Protein Information: protein S100-B; S-100 protein subunit beta; S-100 calcium-binding protein, beta chain; S100 calcium-binding protein, beta (neural)
• UniProt Protein Name: Protein S100-B
• UniProt Gene Name: S100B
• UniProt Entry Name: S100B_HUMAN