Standard Curve (Sample)
(Human KLK5 PicoKine ELISA Kit standard curve)
Human Kallikrein 5/KLK5 ELISA Kit | KLK5 elisa kit
Human Kallikrein 5/KLK5 PicoKine Quick ELISA Kit
Gene Names
KLK5; SCTE; KLKL2; KLK-L2
Reactivity
Human
Applications
ELISA
Synonyms
Kallikrein 5/KLK5; N/A; Human Kallikrein 5/KLK5 PicoKine Quick ELISA Kit; KLK5 elisa kit
Reactivity
Human
Specificity
Natural and recombinant Human KLK5
Assay Type
Sandwich
Samples
Cell culture supernatants, serum, plasma (heparin, EDTA), human milk and saliva
Detection Range
78 pg/ml - 5,000 pg/ml
Sensitivity
<10 pg/ml
Applicable Applications for KLK5 elisa kit
ELISA
Intra-assay Precision
Intra-Assay Precision (Precision within an assay): Three samples of known concentration were tested on one plate to assess intra-assay precision.
Inter-assay Precision
Inter-Assay Precision (Precision across assays): Three samples of known concentration were tested in separate assays to assess inter-assay precision.
Preparation and Storage
Store at 4 degree C for 6 months, at -20 degree C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Standard Curve (Sample)
(Human KLK5 PicoKine ELISA Kit standard curve)
Standard Curve (Sample)
(Human KLK5 PicoKine ELISA Kit standard curve)
Related Product Information for KLK5 elisa kit
Background: Kallikrein gene 5 (KLK5, also known as KLK-L2), located on chromosome 19q13.4, is one of the newly identified members of the kallikrein gene family, which is a subgroup of the serine protease enzyme family. In normal human tissues, KLK5 is highly expressed in skin, mammary gland and testis. KLK5 has been suggested to regulate cell shedding(desquamation) in conjunction with KLK7 and KLK14, given its ability to degrade proteins which form the extracellular component of cell junctions in the stratum corneum. It is proposed that KLK5 regulates this process since it is able to self-activate in addition to activating KLK7 and KLK14.
Principle of the Assay: The Quick Picokine™ Human KLK5 Pre-Coated ELISA (Enzyme-Linked Immunosorbent Assay) kit is a solid phase immunoassay specially designed to measure Human KLK5 in cell culture supernatants, serum, plasma (heparin, EDTA), human milk and saliva. It uses our proprietary Quick ELISA technology. Quick ELISA technology employs capture antibodies conjugated to an affinity tag that is recognized by the polyclonal antibody used to coat our Quick ELISA plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. The kit includes Human KLK5 protein as standards. To measure Human KLK5, add standards and samples to the wells, then add antibody cocktail. Wash the wells with PBS or TBS buffer, and add TMB. TMB is an HRP substrate and will be catalyzed to produce a blue color product, which changes into yellow after adding the acidic stop solution. The absorbance of the yellow product is linearly proportional to Human KLK5 in the sample. Read the absorbance of the yellow product in each well using a plate reader, and benchmark the sample wells' readings against the standard curve to determine the concentration of Human KLK5 in the sample.
Principle of the Assay: The Quick Picokine™ Human KLK5 Pre-Coated ELISA (Enzyme-Linked Immunosorbent Assay) kit is a solid phase immunoassay specially designed to measure Human KLK5 in cell culture supernatants, serum, plasma (heparin, EDTA), human milk and saliva. It uses our proprietary Quick ELISA technology. Quick ELISA technology employs capture antibodies conjugated to an affinity tag that is recognized by the polyclonal antibody used to coat our Quick ELISA plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. The kit includes Human KLK5 protein as standards. To measure Human KLK5, add standards and samples to the wells, then add antibody cocktail. Wash the wells with PBS or TBS buffer, and add TMB. TMB is an HRP substrate and will be catalyzed to produce a blue color product, which changes into yellow after adding the acidic stop solution. The absorbance of the yellow product is linearly proportional to Human KLK5 in the sample. Read the absorbance of the yellow product in each well using a plate reader, and benchmark the sample wells' readings against the standard curve to determine the concentration of Human KLK5 in the sample.
Product Categories/Family for KLK5 elisa kit
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
293
NCBI Official Full Name
Kallikrein-5
NCBI Official Synonym Full Names
kallikrein-related peptidase 5
NCBI Official Symbol
KLK5
NCBI Official Synonym Symbols
SCTE; KLKL2; KLK-L2
NCBI Protein Information
kallikrein-5; kallikrein 5; kallikrein-like protein 2; stratum corneum tryptic enzyme
UniProt Protein Name
Kallikrein-5
UniProt Gene Name
KLK5
UniProt Synonym Gene Names
SCTE; KLK-L2
UniProt Entry Name
KLK5_HUMAN
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Product Notes
The Human KLK5 klk5 (Catalog #AAA126815) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA126815 ELISA Kit recognizes Human KLK5. AAA Biotech's Kallikrein 5/KLK5 can be used in a range of immunoassay formats including, but not limited to, ELISA. Researchers should empirically determine the suitability of the KLK5 klk5 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Kallikrein 5/KLK5, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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