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Application Data
(Tumor protein p53, also known as p53, cellular tumor antigen p53 (UniProt name), phosphoprotein p53, tumor suppressor p53, antigen NY-CO-13, or transformationrelated protein 53 (TRP53), is any isoform of a protein encoded by homologous genes in various organisms, such as TP53 (humans) and Trp53 (mice). TP53 involved in cell cycle regulation as a trans-activator that acts to negatively regulate cell division by controlling a set of genes required for this process. One of the activated genes is an inhibitor of cyclin-dependent kinases. To test the effect of TP53 on cell apoptosis, Jurkat cells were seeded into triplicate wells of 96-well plates at a density of 5,000 cells/well with 1% serum standard 1640 including various concentrations of recombinant human TP53. After incubated for 72h, cells were observed by inverted microscope and cell proliferation was measured by Cell Counting Kit-8 (CCK-8). Briefly, 10uL of CCK-8 solution was added to each well of the plate, then the absorbance at 450nm was measured using a microplate reader after incubating the plate for 1-4 hours at 37?. Proliferation of Jurkat cells after incubation with TP53 for 72h observed by inverted microscope was shown in Figure 1. Cell viability was assessed by CCK-8 (Cell Counting Kit-8) assay after incubation with recombinant TP53 for 72h. The result was shown in Figure 2. It was obvious that TP53 significantly inhibit cell viability of Jurkat cells.)
Tumor Protein p53 Active Protein | P53 active protein
Active Tumor Protein p53 (P53)
Gene Names
TP53; P53; BCC7; LFS1; BMFS5; TRP53
Reactivity
Homo sapiens (Human)
Applications
Activity Assay, Cell Culture
Purity
>90%
Synonyms
Tumor Protein p53; N/A; Active Tumor Protein p53 (P53); TP53; LFS1; TRP53; Li-Fraumeni Syndrome; Cellular tumor antigen p53; Antigen NY-CO-13; Phosphoprotein p53; Tumor suppressor p53; P53 active protein
Host
E. coli
Reactivity
Homo sapiens (Human)
Purity/Purification
>90%
Form/Format
PBS, pH7.4, containing 0.01% SKL, 5% Trehalose.
Concentration
Original Concentration: 80ug/mL (varies by lot)
Applicable Applications for P53 active protein
Activity Assay, Cell Culture
Source
Prokaryotic expression
Residues
Gly108~Lys370
Tags
N-terminal His-tag
Endotoxin Level
<1.0EU per 1ug (determined by the LAL method).
Predicted isoelectric point
9.0
Usage
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex
Preparation and Storage
Storage:
Avoid repeated freeze/thaw cycles.
Store at 2-8 degree C for one month.
Aliquot and store at -80 degree C for 12 months.
Stability Test:
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 degree C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
Avoid repeated freeze/thaw cycles.
Store at 2-8 degree C for one month.
Aliquot and store at -80 degree C for 12 months.
Stability Test:
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 degree C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
Application Data
(Tumor protein p53, also known as p53, cellular tumor antigen p53 (UniProt name), phosphoprotein p53, tumor suppressor p53, antigen NY-CO-13, or transformationrelated protein 53 (TRP53), is any isoform of a protein encoded by homologous genes in various organisms, such as TP53 (humans) and Trp53 (mice). TP53 involved in cell cycle regulation as a trans-activator that acts to negatively regulate cell division by controlling a set of genes required for this process. One of the activated genes is an inhibitor of cyclin-dependent kinases. To test the effect of TP53 on cell apoptosis, Jurkat cells were seeded into triplicate wells of 96-well plates at a density of 5,000 cells/well with 1% serum standard 1640 including various concentrations of recombinant human TP53. After incubated for 72h, cells were observed by inverted microscope and cell proliferation was measured by Cell Counting Kit-8 (CCK-8). Briefly, 10uL of CCK-8 solution was added to each well of the plate, then the absorbance at 450nm was measured using a microplate reader after incubating the plate for 1-4 hours at 37?. Proliferation of Jurkat cells after incubation with TP53 for 72h observed by inverted microscope was shown in Figure 1. Cell viability was assessed by CCK-8 (Cell Counting Kit-8) assay after incubation with recombinant TP53 for 72h. The result was shown in Figure 2. It was obvious that TP53 significantly inhibit cell viability of Jurkat cells.)
Application Data
(Tumor protein p53, also known as p53, cellular tumor antigen p53 (UniProt name), phosphoprotein p53, tumor suppressor p53, antigen NY-CO-13, or transformationrelated protein 53 (TRP53), is any isoform of a protein encoded by homologous genes in various organisms, such as TP53 (humans) and Trp53 (mice). TP53 involved in cell cycle regulation as a trans-activator that acts to negatively regulate cell division by controlling a set of genes required for this process. One of the activated genes is an inhibitor of cyclin-dependent kinases. To test the effect of TP53 on cell apoptosis, Jurkat cells were seeded into triplicate wells of 96-well plates at a density of 5,000 cells/well with 1% serum standard 1640 including various concentrations of recombinant human TP53. After incubated for 72h, cells were observed by inverted microscope and cell proliferation was measured by Cell Counting Kit-8 (CCK-8). Briefly, 10uL of CCK-8 solution was added to each well of the plate, then the absorbance at 450nm was measured using a microplate reader after incubating the plate for 1-4 hours at 37?. Proliferation of Jurkat cells after incubation with TP53 for 72h observed by inverted microscope was shown in Figure 1. Cell viability was assessed by CCK-8 (Cell Counting Kit-8) assay after incubation with recombinant TP53 for 72h. The result was shown in Figure 2. It was obvious that TP53 significantly inhibit cell viability of Jurkat cells.)
Sequence
Sequence
Identfication
(Gene Sequencing (Extract))
Identfication
(Gene Sequencing (Extract))
WB (Western Blot)
(Western Blot; Sample: Recombinant P53, Human; Antibody: Rabbit Anti-Human P53 Ab)
WB (Western Blot)
(Western Blot; Sample: Recombinant P53, Human; Antibody: Rabbit Anti-Human P53 Ab)
SDS-PAGE
(Figure. SDS-PAGESample: Active recombinant P53, Human)
SDS-PAGE
(Figure. SDS-PAGESample: Active recombinant P53, Human)
Product Categories/Family for P53 active protein
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
Predicted Molecular Mass: 33.3kDa
Accurate Molecular Mass: 34kDa as determined by SDS-PAGE reducing conditions.
Accurate Molecular Mass: 34kDa as determined by SDS-PAGE reducing conditions.
NCBI Official Full Name
cellular tumor antigen p53 isoform a
NCBI Official Synonym Full Names
tumor protein p53
NCBI Official Symbol
TP53
NCBI Official Synonym Symbols
P53; BCC7; LFS1; BMFS5; TRP53
NCBI Protein Information
cellular tumor antigen p53
UniProt Protein Name
Cellular tumor antigen p53
UniProt Gene Name
TP53
UniProt Synonym Gene Names
P53
UniProt Entry Name
P53_HUMAN
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Product Notes
The P53 tp53 (Catalog #AAA150104) is an Active Protein produced from E. coli and is intended for research purposes only. The product is available for immediate purchase. The Active Tumor Protein p53 (P53) reacts with Homo sapiens (Human) and may cross-react with other species as described in the data sheet. AAA Biotech's Tumor Protein p53 can be used in a range of immunoassay formats including, but not limited to, Activity Assay, Cell Culture. Researchers should empirically determine the suitability of the P53 tp53 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Tumor Protein p53, Active Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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