Caspase-14 Active Protein | CASP14 active protein

Caspase-14 Protein, Human, Recombinant (His Tag)

Cat. Num. AAA257996

• Purity: >95% as determined by SDS-PAGE
• Synonyms: Caspase-14; N/A; Caspase-14 Protein, Human, Recombinant (His Tag); Human Caspase-14/CASP14 Protein (His Tag); ARCI12 Protein, Human; caspase 14, apoptosis-related cysteine peptidase; CASP14 active protein

• Host: E Coli
• Purity/Purification: >95% as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile 20mM HEPES, 100mM NaCI, 1mM EDTA, 10% Sucrose, 0.1% chaps; pH7.5, 5% trehalose, 5% mannitol, 0.01% Tween80
• Sequence: Ser2-Gln242

• Species: Human
• Predicted N Terminal: Met
• Tag: N-His
• Protein Construction: A DNA sequence encoding the human CASP14 (NP_036246.1)(Ser 2-Gln 242) was expressed, with a polyhistide tag at the N-terminus.
• Reconstitution: It is recommended that the sterile water (200ul) be added to the vial to prepare a stick solution of 0.25mg/mL. Concenration is measured by UV-Vis.
• Bio Activity: Measured by its ability to bind biotinylated Cynomolgus IL18 in a functional ELISA.
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -70°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

SDS-PAGE

Related Product Information for CASP14 active protein

Background: Caspase 14 is a member of the caspase family. Caspases are a kind of cysteine proteinase consisting of a prodomain plus large and small catalytic subunits, that play a central role in cell apoptosis. Caspase 14 possesses an unusually short prodomain and is highly expressed in embryonic tissues but absent from most of the adult tissues except for the skin, which suggests a role in ontogenesis and skin physiology. Unlike the other short prodomain caspases(caspase-3, caspase-6, and caspase-7), Caspase 14 was not processed by multiple death stimuli including activation of members of the tumor necrosis factor receptor family and expression of proapaptotic members of the bcl-2 family. Caspase 14 has been described to be processed and activated by anti-Fas agonist antibody or TNF-related apoptosis inducing ligand in vivo. The expression and processing of this caspase may take part in keratinocyte terminal differentiation, which is essential for the skin barrier.

Product Categories/Family for CASP14 active protein

EGFR Signaling Pathway

References

Hu SM, et al. (1998) Caspase-14 is a novel developmentally regulated protease. The journal of biological chemistry. 273: 29648-53.Marc Van De Craen, et al. (1998) Identification of a new caspase homologue: caspase-14. Cell death differ. 5(10): 838-46.

NCBI and Uniprot Product Information

• NCBI GI #: 6912286
• NCBI GeneID: 23581
• NCBI Accession #: NP_036246.1
• NCBI GenBank Nucleotide #: NM_012114.2
• UniProt Accession #: P31944; O95823; Q3SYC9
• Molecular Weight: The recombinant human CASP14 consisting of 248 amino acids and has a calculated molecular mass of 28.5 kDa. The apparent molecular mass of the protein is approximately 30 kDa in SDS-PAGE under reducing conditions.
• NCBI Official Full Name: caspase-14
• NCBI Official Synonym Full Names: caspase 14, apoptosis-related cysteine peptidase
• NCBI Official Symbol: CASP14
• NCBI Protein Information: caspase-14; CASP-14; apoptosis-related cysteine protease; caspase 14, apoptosis-related cysteine protease
• UniProt Protein Name: Caspase-14
• UniProt Gene Name: CASP14
• UniProt Synonym Gene Names: CASP-14
• UniProt Entry Name: CASPE_HUMAN