CD97 recombinant protein

Recombinant Human CD97 Protein (His tag)(Active)

Cat. Num. AAA173549

• Gene Names: ADGRE5; CD97; TM7LN1
• Purity: > 85 % as determined by SDS-PAGE
• Synonyms: CD97; N/A; Recombinant Human CD97 Protein (His tag)(Active); TM7LN1, CD97; CD97 recombinant protein

• Host: HEK293 cells
• Purity/Purification: > 85 % as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile PBS, pH7.4, 5% Trehalose, 5% Mannitol, 0.01 % Tween-80.
• Sequence Length: 742

• Species: Human
• Predicted N Terminal: Gln 21
• Mol_Mass: 42.6 kDa
• AP_Mol_Mass: 60-70kDa
• Tag: C-His
• Bio_activity: Inmobilized recombinant human CD97 at 10 ug/mL (100 uL/well) can bind recombinant human human CD55 at a linear range of 0.46-30 ug/mL.
• Reconstitution: We recommend centrifuging the vial before opening to bring the contents to the bottom. Do not mix by vortex.; ; It is recommended that sterile water be added to the vial to prepare a stock solution of 0.25 mg/mL. Concentration is measured by UV-Vis.
• Background: The cluster of differentiation (CD) system is commonly used as cell markers in immunophynotyping. Different kinds of cells in the immune system can be identified through the surface CD molecules which associating with the immune function of the cell. There are more than 320 CD unique clusters and subclusters have been identified. Some of the CD molecules serve as receptors or ligands important to the cell through initiating a signal cascade which then alter the behavior of the cell. Some CD proteins do not take part in cell signal process but have other functions such as cell adhesion. The CD97 is a receptor predominantly expressed in leukocytes and belongs to a new group of seven-span transmembrane molecules, which is also designed EGF-TM7 family. The family members are characterized by an extended extracellular region with several N-terminal epidermal growth factor-like domains two of which contain a calcium binding site. Muture CD 97 has two noncovalently associated subunits and is composed of a large extracellular protein (CD97 alpha) and a seven-membrane spanning protein (CD97 beta). CD97 is considered as a defining feature of G protein-coupled receptors. The effects that lymphocytes and erythrocytes adere to CD97-transfected COS cells suggest that CD97 has the ability to bind cellular ligands. CD97 alpha has three alternatively spliced isforms that are related to the calium binding EGF-like repeats in the microfibril protein fibrillin. Leukocytes strongly positive for CD97 are concentrated at sites of inflammation relative to CD97 expression in normal lymphoid tissues.
• Preparation and Storage: Generally, lyophilized proteins are stable for up 12 months when stored at -20°C to -80°C. Reconstituted protein solution can be stored at -20°C to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 day. Aliquots of reconstituted samples are stable at <-20°C for 3 months .

SDS-PAGE

Related Product Information for CD97 recombinant protein

A DNA sequence encoding the first 398 amino acids (Met 1-Gln 398) of human CD97 isoform 2 (NP_001775.2) extracellular domain was fused with a polyhistidine tag at the C-terminus.

NCBI and Uniprot Product Information

• NCBI GI #: 17978489
• NCBI GeneID: 976
• NCBI Accession #: NP_001775.2
• NCBI GenBank Nucleotide #: NM_001784.4
• UniProt Accession #: P48960; O00718; O76101; Q8NG72; Q8TBQ7; A8K7Z4; B2RBJ9
• NCBI Official Full Name: CD97 antigen isoform 2 preproprotein
• NCBI Official Synonym Full Names: adhesion G protein-coupled receptor E5
• NCBI Official Symbol: ADGRE5
• NCBI Official Synonym Symbols: CD97; TM7LN1
• NCBI Protein Information: CD97 antigen
• UniProt Protein Name: CD97 antigen
• UniProt Gene Name: CD97