PD-L1 Recombinant Protein | CD274 recombinant protein

PD-L1 Protein, Mouse, Recombinant (ECD, hFc Tag)

Cat. Num. AAA257463

• Gene Names: Cd274; B7h1; Pdl1; Pdcd1l1; Pdcd1lg1; A530045L16Rik
• Purity: >95 % as determined by SDS-PAGE
• Synonyms: PD-L1; N/A; PD-L1 Protein, Mouse, Recombinant (ECD, hFc Tag); Mouse PD-L1/B7-H1/CD274 Protein (ECD, Fc Tag); CD274 molecule; A530045L16Rik Protein, Mouse; B7h1 Protein, Mouse; Pdcd1l1 Protein, Mouse; Pdcd1lg1 Protein, Mouse; Pdl1 Protein, Mouse; CD274 recombinant protein

• Host: HEK293 Cells
• Purity/Purification: >95 % as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile PBS, pH7.4. Normally 5%-8% trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
• Sequence Positions: 459aa
• Sequence: Met1-His239

• Species: Mouse
• Tag: hFc
• Endotoxin: <1.0EU per ug protein as determined by the LAL method
• Predicted N Terminal: Phe19
• Protein Construction: |A DNA sequence encoding the mouse Cd274 (NP_068693.1) (Met1-His239) was expressed with the Fc region of human IgG1 at the C-terminus
• Activity: 1. Measured by its binding ability in a functional ELISA.; 2. Immobilized human PD1-His (Cat:10377-H08H) at 10ug/mL (100uL/well) can bind mouse CD274-Fc (Cat:50010-M02H). The EC50 of mouse CD274-Fc is 0.1-0.6ug/mL.
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -20 degree C to -80 degree C. Store it under sterile conditions at -20 degree C to -80 degree C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.; Shipping: They are shipped out with blue ice pack

Application Data

(Measured by its binding ability in a functional ELISA. Immobilized human PD1-His (Cat: 10377-H08H) at 10 ug/mL (100 uL/well) can bind mouse CD274-Fc (Cat: 50010-M02H), the EC50 of mouse CD274-Fc is 0.1-0.6ug/mL.)

Application Data

Related Product Information for CD274 recombinant protein

Programmed death-1 ligand-1 (PD-L1, CD274, B7-H1) has been identified as the ligand for the immunoinhibitory receptor programmed death-1(PD1/PDCD1) and has been demonstrated to play a role in the regulation of immune responses and peripheral tolerance. PD-L1/B7-H1 is a member of the growing B7 family of immune molecules and this protein contains one V-like and one C-like Ig domain within the extracellular domain, and together with PD-L2, are two ligands for PD1 which belongs to the CD28/CTLA4 family expressed on activated lymphoid cells. By binding to PD1 on activated T-cells and B-cells, PD-L1 may inhibit ongoing T-cell responses by inducing apoptosis and arresting cell-cycle progression. Accordingly, it leads to growth of immunogenic tumor growth by increasing apoptosis of antigen specific T cells and may contribute to immune evasion by cancers. PD-L1 thus is regarded as promising therapeutic target for human autoimmune disease and malignant cancers.

Product Categories/Family for CD274 recombinant protein

Cancer Drug Targets

References

Iwai Y, et al. (2002) Involvement of PD-L1 on tumor cells in the escape from host immune system and tumor immunotherapy by PD-L1 blockade. Proc Natl Acad Sci U S A. 99(19): 12293-7.Ghebeh H, et al. (2006) The B7-H1 (PD-L1) T lymphocyte-inhibitory molecule is expressed in breast cancer patients with infiltrating ductal carcinoma: correlation with important high-risk prognostic factors. Neoplasia. 8(3): 190-8.Salih HR, et al. (2006) The role of leukemia-derived B7-H1 (PD-L1) in tumor-T-cell interactions in humans. Exp Hematol. 34(7): 888-94.Wilcox RA, et al. (2009) B7-H1 (PD-L1, CD274) suppresses host immunity in T-cell lymphoproliferative disorders. Blood. 114(10): 2149-58.Ruggiero A, et al. (2009) Crystal structure of PD-L1, a ribosome inactivating protein from Phytolacca dioica L. leaves with the property to induce DNA cleavage. Biopolymers. 91(12): 1135-42.

NCBI and Uniprot Product Information

• NCBI GI #: 11230798
• NCBI GeneID: 60533
• NCBI Accession #: NP_068693.1
• NCBI GenBank Nucleotide #: NM_021893.3
• UniProt Accession #: Q9EP73
• NCBI Official Full Name: programmed cell death 1 ligand 1
• NCBI Official Synonym Full Names: CD274 antigen
• NCBI Official Symbol: Cd274
• NCBI Official Synonym Symbols: B7h1; Pdl1; Pdcd1l1; Pdcd1lg1; A530045L16Rik
• NCBI Protein Information: programmed cell death 1 ligand 1; B7 homolog 1; PDCD1 ligand 1; programmed death ligand 1
• UniProt Protein Name: Programmed cell death 1 ligand 1
• UniProt Gene Name: Cd274
• UniProt Synonym Gene Names: B7h1; Pdcd1l1; Pdcd1lg1; Pdl1; PD-L1; PDCD1 ligand 1; Programmed death ligand 1; B7-H1
• UniProt Entry Name: PD1L1_MOUSE