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product-image-AAA80915_SC15.png Standard Curve (Sample)

Bovine Epithelial growth factor (EGF) ELISA Kit | EGF elisa kit

Bovine Epithelial growth factor, EGF ELISA Kit

Gene Names
EGF; URG; HOMG4
Reactivity
Bovine
Synonyms
Epithelial growth factor (EGF); N/A; Bovine Epithelial growth factor, EGF ELISA Kit; Bovine Epithelial growth factor (EGF) ELISA Kit; HOMG4; URG; beta-urogastrone; epidermal growth factor; EGF elisa kit
Ordering
Reactivity
Bovine
Specificity
This assay has high sensitivity and excellent specificity for detection of mouse EFEMP1. No significant cross-reactivity or interference between mouse EFEMP1 and analogues was observed.
Sequence Length
1166
Samples
Serum, Plasma, Tissue Homogenates, Cell Lysates
Assay Type
Quantitative Sandwich
Detection Range
31.25 pg/ml-2000 pg/ml
Sensitivity
7.81 pg/ml.
Intra-assay Precision
Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess
Inter-assay Precision
Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
Preparation and Storage
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Standard Curve (Sample)

product-image-AAA80915_SC15.png Standard Curve (Sample)
Related Product Information for EGF elisa kit
Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for EFEMP1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any EFEMP1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for EFEMP1 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of EFEMP1 bound in the initial step. The color development is stopped and the intensity of the color is measured.

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
133,994 Da
NCBI Official Full Name
pro-epidermal growth factor isoform 2 preproprotein
NCBI Official Synonym Full Names
epidermal growth factor
NCBI Official Symbol
EGF
NCBI Official Synonym Symbols
URG; HOMG4
NCBI Protein Information
pro-epidermal growth factor; beta-urogastrone
UniProt Protein Name
Pro-epidermal growth factor
UniProt Gene Name
EGF
UniProt Synonym Gene Names
EGF
UniProt Entry Name
EGF_HUMAN

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Product Notes

The Bovine EGF egf (Catalog #AAA80915) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA80915 ELISA Kit recognizes Bovine EGF. It is sometimes possible for the material contained within the vial of "Epithelial growth factor (EGF), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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