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product-image-AAA20987_SDS_PAGE2.jpg SDS-PAGE

Aspartate Beta Hydroxylase Recombinant Protein | ASPH recombinant protein

Recombinant Aspartate Beta Hydroxylase (ASPH)

Gene Names
ASPH; AAH; BAH; HAAH; JCTN; FDLAB; junctin; CASQ2BP1
Applications
SDS-Page, Western Blot
Purity
> 97%
Synonyms
Aspartate Beta Hydroxylase; N/A; Recombinant Aspartate Beta Hydroxylase (ASPH); ASPH recombinant protein
Ordering
For Research Use Only!
Host
E.coli
Purity/Purification
> 97%
Form/Format
20mM Tris, 150mM NaCl, pH8.0, containing 0.01% SKL, 5% Trehalose.
Concentration
750 ug/mL (varies by lot)
Sequence
RSLYNVNGL KAQPWWTPKE TGYTELVKSL ERNWKLIRDE GLAVMDKAKG LFLPEDENLR EKGDWSQFTL WQQGRRNENA CKGAPKTCTL LEKFPETTGC RRGQIKYSIM HPGTHVWPHT GPTNCRLRMH LGLVIPKEGCKIRCANETKT WEEGKVLIFD DSFEHEVWQD ASSFRLIFIV DVWHPELTPQ QRRRPSSNLA RAPPPPPLRS GC
Applicable Applications for ASPH recombinant protein
Positive Control; Immunogen; SDS-PAGE; Western Blot (WB)

(May be suitable for use in other assays to be determined by the end user.)
Organism Species
Homo sapiens (Human)
Source
Prokaryotic expression
Residues
Arg562~Arg753+RPSSNLARAPPPPPLRSGC
Tags
N-terminal His Tag
Subcellular Location
Endoplasmic reticulum lumen
Traits
Freeze-dried powder
Predicted isoelectric point
9.1
Usage
Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL.
Do not vortex.
Preparation and Storage
Storage:
Avoid repeated freeze/thaw cycles.
Store at 2-8ºC for one month.
Aliquot and store at -80ºC for 12 months.

Stability Test:
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

SDS-PAGE

product-image-AAA20987_SDS_PAGE2.jpg SDS-PAGE

Gene Sequencing (Extract)

product-image-AAA20987_SEQUENCE.jpg Gene Sequencing (Extract)

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
444
Molecular Weight
Predicted Molecular Mass: 28.1kDa
Accurate Molecular Mass: 27kDa as determined by SDS-PAGE reducing conditions.
NCBI Official Full Name
aspartate beta-hydroxylase, partial
NCBI Official Synonym Full Names
aspartate beta-hydroxylase
NCBI Official Symbol
ASPH
NCBI Official Synonym Symbols
AAH; BAH; HAAH; JCTN; FDLAB; junctin; CASQ2BP1
NCBI Protein Information
aspartyl/asparaginyl beta-hydroxylase
UniProt Protein Name
Aspartyl/asparaginyl beta-hydroxylase
UniProt Gene Name
ASPH
UniProt Synonym Gene Names
BAH; ASP beta-hydroxylase
UniProt Entry Name
ASPH_HUMAN

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Product Notes

The ASPH asph (Catalog #AAA20987) is a Recombinant Protein produced from E.coli and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's Aspartate Beta Hydroxylase can be used in a range of immunoassay formats including, but not limited to, Positive Control; Immunogen; SDS-PAGE; Western Blot (WB)

(May be suitable for use in other assays to be determined by the end user.). Researchers should empirically determine the suitability of the ASPH asph for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: RSLYNVNGL KAQPWWTPKE TGYTELVKSL ERNWKLIRDE GLAVMDKAKG LFLPEDENLR EKGDWSQFTL WQQGRRNENA CKGAPKTCTL LEKFPETTGC RRGQIKYSIM HPGTHVWPHT GPTNCRLRMH LGLVIPKEGC KIRCANETKT WEEGKVLIFD DSFEHEVWQD ASSFRLIFIV DVWHPELTPQ QRRRPSSNLA RAPPPPPLRS GC. It is sometimes possible for the material contained within the vial of "Aspartate Beta Hydroxylase, Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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